简单合成的pH适应性酸盐过氧化酶类纳米酶的一步酶-纳米酶级联生物传感
Huifang Zhang1,2, Yuhang Zhang2, Xiang Xu2
1College of Chemistry and Chemical Engineering, Key Laboratory of Jiangxi Province for Environment and Energy Catalysis, Nanchang University, Nanchang 330031, China.
Analytical chemistry
|February 2, 2026
概括
一种新型的纳米酶,铜合的氧化伊米达酸框架-90 (Cu/Zn-ZIF-90),使得适应pH的酶级联能够用于敏感的小分子检测. 这一突破克服了以前的局限性,为更简单,更有效的生物传感器铺平了道路.
科学领域:
- 纳米材料科学 科学 纳米材料科学
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 纳米酶在酶-纳米酶级联系统中提供优势,用于小分子检测.
- 挑战包括pH不相容性和复杂的纳米酶制备,阻碍级联性能.
研究的目的:
- 开发一种易于合成的,适应pH的纳米酶,用于酶-纳米酶级联系统.
- 为了克服级反应中的pH不匹配问题,并使单步生物感知成为可能.
主要方法:
- 在10分钟内轻松合成合铜的焦化物伊米达酸框架-90 (Cu/Zn-ZIF-90).
- 描述Cu/Zn-ZIF-90作为一种类似酸盐过氧化酶 (APX) 的纳米酶,对H2O2有很高的亲和力.
- 集成类似APX的Cu/Zn-ZIF-90与胆氧化酶,用于一级级联列光生物传感器.
主要成果:
- /Zn-ZIF-90在中性pH下表现出最佳活性,解决了pH不匹配问题.
- 该纳米酶与其他纳米酶相比,对H2O2具有较高的亲和力,促进中间转化.
- 开发的生物传感器实现了广泛的线性范围 (1-1000μM) 和低检测极限 (0.85μM) 胆检测.
结论:
- 模仿APX的纳米酶对于克服级pH障碍至关重要.
- 开发的Cu/Zn-ZIF-90纳米酶可实现高效的一步小分子生物传感.
- 这种方法在分析复杂的食品矩阵方面具有强大的应用性.
相关概念视频
Intracellular Signaling Cascades
53.6K
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
53.6K
Enzymes
94.7K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
94.7K
Rab Cascades
3.6K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
3.6K
Transfer RNA Synthesis
13.3K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
13.3K
Amplifying Signals via Enzymatic Cascade
18.5K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
18.5K
MAPK Signaling Cascades
8.4K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.4K


