在VrERF088中的自然变异调节了芽期间的干旱耐受性
Xi Zhang1, Jinyang Liu1, Shicong Li1
1Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
概括
干旱压力严重影响豆种子的发芽. 在VrERF088基因中的特定突变.
科学领域:
- 植物遗传学 植物遗传学
- 农业科学 农业科学
- 分子生物学分子生物学
背景情况:
- 干旱压力对全球作物生产构成重大威胁,特别是影响种子发芽和建立.
- 豆 (Vigna radiata) 是一种重要的豆类作物,在早期生长阶段容易受到干旱的影响.
研究的目的:
- 为了确定控制干旱压力下的芽的遗传因素.
- 开发用于选择耐旱的豆品种的分子标记物.
主要方法:
- 414个豆生殖质的全基因组重新排序,以确定单核酸多态 (SNP).
- 全基因组关联研究 (GWAS) 以确定在发芽期间耐干旱的定量特征位置 (QTL).
- 开发和验证竞争性等位基特异性PCR (KASP) 标记物.
- 在Arabidopsis thaliana*中进行基因表达分析和功能验证.
主要成果:
- 确定了4,875,142个高质量的SNP和与干旱压力下的种子发芽相关的显著QTL.
- 为干旱耐受性选择开发了功能性的KASP标志物.
- 发现了VrERF088促进体的ABA反应元件 (ABRE) 的突变.
- 证明了VraBI5通过ABRE动机激活Hap1促销器.
- 在Arabidopsis thaliana中,VrERF088的过度表达导致对ABA过敏,干旱耐受性降低.
结论:
- VrERF088促进体的ABRE动机的突变与干旱压力下更高的发芽率有关.
- VrERF088在ABA信号和干旱响应中起着至关重要的作用.
- 鉴定的遗传资源和标记物可以帮助培育抗旱的豆作物.
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