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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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What is Genetic Engineering?00:49

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Overview
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Classifying Matter by Composition03:35

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Matter: Pure Substances and Mixtures
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures. 
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Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
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相关实验视频

Updated: Feb 4, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
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在Euglena gracilis中使用基因组编辑的工程 Ester组成.

Sakura Nagamine1, Rikuto Oishi1, Masami Nakazawa2

  • 1Department of Applied Biochemistry, Faculty of Agriculture, Osaka Metropolitan University, Sakai, Osaka, Japan.

Methods in molecular biology (Clifton, N.J.)
|February 2, 2026
PubMed
概括
此摘要是机器生成的。

这项研究详细介绍了Euglena gracilis的CRISPR/Cas9基因组编辑协议,以修改 Ester的生产. 这种方法使E. gracilis的稳定基因工程能够用于合成生物学和生物制造应用.

关键词:
厌氧代谢 厌氧代谢这就是CRISPR/Cas9的作用.优格伦娜·格拉西利斯 (Euglena gracilis) 是一个有趣的植物.脂肪酸β-氧化 脂肪酸β-氧化基因组编辑 基因组编辑功能丧失 - 功能丧失.代谢修饰是一种代谢修饰.有针对性的基因淘汰赛.Ester 的合成

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科学领域:

  • 合成生物学 合成生物学
  • 代谢工程是代谢工程.
  • 基因组学就是基因组学.

背景情况:

  • 基因组编辑的进步使得非模型生物的代谢工程成为可能,例如Euglena gracilis.
  • 已经建立了CRISPR/Cas9和CRISPR/Cas12a系统,用于E. gracilis的基因组编辑.

研究的目的:

  • 为基于CRISPR/Cas9的E. gracilis.基因组编辑提供详细的协议.
  • 为了在无氧条件下使 Ester 组成能够稳定地改变.

主要方法:

  • 利用CRISPR/Cas9技术在逆转β-氧化途径中准关键酶.
  • 产生淘汰突变物来改变E. gracilis的 Ester链长度.

主要成果:

  • 成功生成了E. gracilis的淘汰突变,改变了 Ester 链的长度.
  • 建立了一种可复制和稳定的方法,用于对E. gracilis代谢进行基因改造.

结论:

  • 开发的CRISPR/Cas9协议促进了E. gracilis代谢的稳定基因修饰.
  • 这种方法支持使用E. gracilis作为合成生物学和生物制造的绿色底盘,有潜力用于敲门策略.