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Updated: Feb 4, 2026

The Soft Agar Colony Formation Assay
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殖民地形成试验检测LncRNA-knockdown骨髓瘤细胞的繁殖能力

Xinyu Zhang1, Junli Chang1, Binghan Yan1

  • 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education.

Journal of visualized experiments : JoVE
|February 2, 2026
PubMed
概括

这项研究提出了一种可靠的殖民地形成测定方案,以评估长非编码RNA (lncRNA) 对骨髓瘤细胞增殖的影响. 抑制SNHG6 lncRNA显著降低了骨髓瘤细胞增殖,突出了它的作用.

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lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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