一个向内质网膜的近红外光探针用于对低酸进行选择性成像
Leqin Cheng1, Mengjuan Shi1, Sijie Li2
1School of Chemistry and Pharmaceutical Engineering, Jilin University of Chemical Technology, Jilin 132022, Jilin, China.
Bioorganic & medicinal chemistry
|February 2, 2026
概括
研究人员开发了一种新的近红外光探针,XB-CTs,用于检测内网膜 (ER) 中的低酸 (HClO). 这个工具有助于理解ER氧化应激和癌症和神经退行等相关疾病.
科学领域:
- 生物医学工程 生物医学工程
- 化学生物学 化学生物学
- 分子成像学分子成像学
背景情况:
- 低酸 (HClO) 对于内细胞网膜 (ER) 恒常状态至关重要.
- HClO的不平衡与神经退行性疾病和癌症有关.
- 在ER中精确检测HClO对于了解细胞过程至关重要.
研究的目的:
- 开发一种"启动"的近红外 (NIR) 光探头,用于在ER中检测特定的HClO.
- 为了实现实时成像HClO动态在活细胞和体内.
- 为研究ER氧化应激提供一个工具.
主要方法:
- 设计和合成的XB-CTs探针使用二二丁-hemicyanine染料.
- 纳入一个N,N-二甲基thiocarbamate组用于HClO特异性和一个p-toluenesulfonamide组用于ER向.
- 在实验室 (灵敏度,反应时间) 和活细胞和小鼠中评估探针性能.
主要成果:
- XB-CTs探头在735nm处表现出特定的"启动"NIR光.
- 实现了低检测极限 (0.141μM) 与快速响应时间 (1秒).
- 在活细胞和小鼠中证明了成功的ER向和高信号噪声比成像.
结论:
- XB-CTs是一种敏感和特定的探针,用于在ER中检测HClO.
- 该探测器可实现ER氧化应激的实时成像.
- 这种工具在生物医学研究中具有潜在的应用,用于研究与ER功能障碍相关的疾病.
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