目标部位和非目标部位的电阻机制都在Silene conoidea L中赋予了 mesosulfuron-methyl电阻
Xinhui Xue1, Hailan Cui2, Shen'ao Hu1
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Plant Protection, Northeast Agricultural University, Harbin 150030, China.
沉默的 conoidea 已经进化了对中硫甲基除草剂的耐药性. 这种耐药性是由于ALS基因的突变以及CYP450s和GSTs等解毒酶的活性增加.
科学领域:
- 农业科学 农业科学
- 植物生物学 植物生物学
- 杂草科学 杂草科学
背景情况:
- Silene conoidea L. 是在小麦田中普遍存在的杂草.
- 抑制乙酸乳酸合成酶 (ALS) 的除草剂,如硫甲基,是主要的控制方法.
- 了解除草剂耐药性机制对于可持续农业至关重要.
研究的目的:
- 为了研究一种耐美苏甲基的Silene conoidea群体的耐药性机制.
- 确定除草剂耐药性的遗传和生化基础.
- 探索目标站点和非目标站点抵抗的参与.
主要方法:
- 对抗性 (R) 和敏感性 (S) S. conoidea 种群的比较分析.
- 在体外ALS酶活性测定.
- ALS基因测序和分子对接.
- 基因表达分析 (qPCR,转录组).
- 使用细胞染色体P450和谷氨S转移酶抑制剂进行抑制试验.
主要成果:
- R群体对甲基硫 (RI=18.87) 呈现出高耐药性,以及对其他ALS抑制剂的交叉耐药性.
- 确定了ALS基因中的W574L突变,削弱了除草剂的结合.
- 在R群体中观察到ALS酶活性升高和ALS基因表达增加.
- 证实了细胞染色体P450s和谷氨S转移酶在耐药性的参与.
- 检测到ABC载体,CYP450和GST基因的升级.
结论:
- 这项研究报告了S. conoidea.中第一次出现了中硫甲基耐药性的情况.
- 耐药性是由目标部位 (ALS基因突变) 和非目标部位 (增强解毒) 机制的组合所赋予的.
- 这些发现凸显了杂草中除草剂耐药性的复杂演变.
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