打破权衡:Trichoderma效应剂Tahfb1通过Hsp70L通过Hsp70L的双重目标增长和防御途径
Yaping Yin1, Yuhang Fu1, He Wu1
1Sanya Institute of Breeding and Multiplication, Engineering Center of Agricultural Microbial Preparation Research and Development of Hainan/School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, PR China.
Pesticide biochemistry and physiology
|February 2, 2026
概括
番茄 (Trichoderma asperellum) 增强了番茄的生长和抗病能力. 一种关键蛋白质Tahfb1与Hsp70L相互作用,通过调节植物激素通路来促进根部发育和免疫力.
科学领域:
- 植物与微生物的相互作用
- 分子植物病理学 分子植物病理学
- 植物生理学 植物生理学
背景情况:
- 像Trichoderma这样的有益微生物调节了植物的生长和防御.
- 关键的分子机制和信号分子尚未完全理解.
研究的目的:
- 阐明Trichoderma asperellum增强番茄生长和对Botrytis cinerea耐药性的分子机制.
- 确定关键的信号分子和参与这种有益相互作用的蛋白质相互作用.
主要方法:
- 相互作用转录组学以识别差异表达的基因.
- 基因编辑CRISPR/Cas9和基因过度表达/沉默 (RNAi).
- 生物化学分析包括酵母二杂交 (Y2H),共免疫沉 (Co-IP) 和双分子光补充 (BiFC).
- 植物生长和抗病能力的定量分析.
主要成果:
- 特里科德尔玛阿斯佩雷勒 (Trichoderma asperellum) 增加了Tahfb1的调节,Tahfb1是一种II类水蛋白,增加了156倍.
- 外源的Tahfb1蛋白和Tahfb1的过度表达促进了横向根的生长,并减少了疾病病变的大小.
- 淘汰Tahfb1破坏了根部的发育,增加了对Botrytis cinerea的敏感性.
- Tahfb1与番茄热冲击蛋白Hsp70L直接相互作用.
- 沉默Hsp70L降低了Tahfb1诱导的增长促进和增强的疾病易感性.
- Tahfb1调节了奥素和莉酸/乙烯信号通路.
结论:
- 确定Tahfb1-Hsp70L模块是Trichoderma-番茄相互作用的关键调解器.
- Tahfb1与Hsp70L协同作用,增强植物生长和免疫力.
- 这种相互作用涉及关键植物激素信号通路的调节,为植物防御和生长调节提供了洞察力.
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