对DNA与TURKOVAC和Pfizer-BioNTech疫苗相互作用的电化学和光谱测量调查:对生物传感器敏感性的影响
Mehmet Aslan1, Abdulkadir Levent2
1Batman, Science and Art Centre, 72100, Batman, Turkey.
Analytica chimica acta
|February 2, 2026
概括
使用一次性笔石墨电极的新型电化学传感器被开发用于分析TURKOVAC和Pfizer-BioNTech等SARS-CoV-2疫苗. 这种绿色分析方法有效研究疫苗-DNA相互作用,对于质量控制和未来疫苗开发至关重要.
科学领域:
- 电化学 电化学 电化学
- 生物传感器技术技术
- 疫苗分析 疫苗分析
背景情况:
- 随着COVID-19的流行,疫苗的开发迅速发展 (例如TURKOVAC,Pfizer-BioNTech).
- 了解疫苗的电化学行为和生物分子相互作用对于安全性和有效性至关重要.
- 需要具有成本效益,灵敏和绿色的分析方法来对疫苗进行表征.
研究的目的:
- 为SARS-CoV-2疫苗开发一个简单,具有成本效益的电化学生物传感平台.
- 为了研究TURKOVAC和瑞-BioNTech疫苗的电化学行为和DNA相互作用.
- 为疫苗分析和质量控制提供绿色分析方法.
主要方法:
- 在一次性笔石墨电极上进行微分脉冲电压测量 (DPV).
- 电化学表征包括循环电压测量和电化学阻抗光谱学.
- 紫外线可见光谱光度计用于疫苗-DNA相互作用的补充分析.
主要成果:
- 在TURKOVAC (+0.90 V) 和瑞生物科技 (+0.92 V) 疫苗中发现了明显的氧化峰值.
- 传感器证实了可重现的性能和疫苗配方的成功表征.
- DPV和光谱测量显示,这两种疫苗都具有热力学稳定的DNA结合,验证了传感器的灵敏度和可靠性.
结论:
- 首次展示一次性笔石墨电极,用于同时对SARS-CoV-2疫苗进行电化学和光谱分析.
- 开发的平台为疫苗质量控制提供了一种绿色,可持续和一次性方法.
- 促进生物传感器开发和理解分子水平的疫苗-生物分子相互作用的潜力,用于未来的疫苗设计.
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