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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Light Acquisition02:16

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In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
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Predators consume prey for energy. Predators that acquire prey and prey that avoid predation both increase their chances of survival and reproduction (i.e., fitness). Routine predator-prey interactions elicit mutual adaptations that improve predator offenses, such as claws, teeth, and speed, as well as prey defenses, including crypsis, aposematism, and mimicry. Thus, predator-prey interactions resemble an evolutionary arms race.
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The most common types of radioactivity are α decay, β decay, γ decay, neutron emission, and electron capture.
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Photosynthesis is a multipart, biochemical process that occurs in plants as well as in some bacteria. It captures carbon dioxide and solar energy to produce glucose. Glucose stores chemical energy in the form of carbohydrates. The overall biochemical formula of photosynthesis is 6 CO2 + 6 H2O + Light energy → C6H12O6 + 6 O2. Photosynthesis releases oxygen into the atmosphere and is largely responsible for maintaining the Earth’s atmospheric oxygen content.
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相关实验视频

Updated: Feb 4, 2026

Laser Capture Microdissection of Mammalian Tissue
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激光捕捉微解剖,然后进行ATAC-Seq.

Celia L Hartmann1,2, Joachim L Schultze2,3,4, Marc D Beyer1,2,3

  • 1Immunogenomics & Neurodegeneration, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) e.V, Bonn, Germany.

Methods in molecular biology (Clifton, N.J.)
|February 2, 2026
PubMed
概括

这项研究详细介绍了与ATAC-seq (LCM-ATAC-seq) 结合的激光捕获微解剖的协议. 这种方法允许在新鲜冷的组织中对染色质景观进行空间分析,捕捉到单个核.

关键词:
ATAC-seqq 的使用情况.染色体的可访问性 染色体的可访问性激光捕获微解剖的微解剖方法形态上的染色是形态上的染色.空间奥米克斯 空间奥米克斯

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相关实验视频

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 激光捕获微解剖 (LCM) 从组织切片中分离出特定的细胞.
  • 空间分辨的omics应用程序需要精确的样本采集.
  • 了解特定组织区域的染色质可访问性至关重要.

研究的目的:

  • 为与ATAC-seq (LCM-ATAC-seq) 结合的LCM提供详细的协议.
  • 为了能够在新鲜冷的组织样本中对染色质景观进行空间分析.
  • 在形态上定义的细胞群体中表征染色质状态.

主要方法:

  • 基于卡拉罗等人的LCM-ATAC-seq的详细协议. (细胞复制方法 3:100598,2023).
  • 有效地捕获生物材料,直到单个核.
  • 整合与形态染色用于细胞群体表征.

主要成果:

  • 成功实施LCM-ATAC-seq用于空间表观基因组分析.
  • 从新鲜冷的组织中高效地捕获核.
  • 在特定的,形态上识别的细胞类型中,染色质状态的表征.

结论:

  • LCM-ATAC-seq是空间色素景观分析的有效方法.
  • 该协议允许对新鲜冷组织进行详细的表观基因组分析.
  • 这种技术有助于研究细胞类型特定的染色质状态在它们的原生环境中.