全基因组协会研究确定了一种α/β酶基因 LeABH 增强Lentinula edodes对Trichoderma的抗性
Yan Xia1, Wenbing Gong2, Liping Yang1
1State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Hubei, China.
Environmental microbiology
|February 2, 2026
概括
绿病威胁着西竹 (Lentinula edodes) 的种植. 研究人员开发了一种新方法来评估耐药性,并确定了一个关键基因LeABH,显著提高了shiitake的抗病能力.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 遗传学 是一个遗传学.
背景情况:
- 由Trichoderma spp.引起的绿色菌病. 对Lentinula edodes (石竹) 种植构成重大威胁,导致大幅度的产量损失.
- 有效的耐药性评估和育种策略对于减轻这些损失至关重要.
研究的目的:
- 开发一种可量化的,基于图像的方法来评估Lentinula edodes对Trichoderma的耐药性.
- 确定遗传因素,包括特定的基因和单核酸多态性 (SNP),与L. edodes.中Trichoderma耐药性相关.
- 为了功能验证候选基因在疾病耐药性中的作用.
主要方法:
- 开发了一种基于Trichoderma感染率 (TIR) 的图像分析方法来评估耐药性.
- 在使用量化TIR的石竹关联小组上进行了全基因组关联研究 (GWAS).
- 通过基因过度表达实验,通过优先级和功能验证的候选基因,包括α/β酶基因 (LeABH).
主要成果:
- 确定了239个SNP和245个与Trichoderma耐药性相关的附近基因.
- 在染色体3,9和10上发现了三个稳定的SNP,与多个TIR特征相关.
- 过度表达LeABH显著增强了L. edodes对T. harzianum和T. atroviride的抵抗力,同时也抑制了真菌的结合.
结论:
- 证实了LeABH作为一个关键的因果基因,在L. edodes.中赋予对Trichoderma的耐药性.
- 这项研究提供了宝贵的遗传资源和对L. edodes耐药性的遗传基础的见解.
- 开发的评估方法和识别的遗传标记可以帮助分子标记器辅助的疾病耐药的石竹菌株的繁殖.
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