研究人类内源逆转录病毒E env转录在子宫癌中的表达水平
Shaghayegh Jahanshahi1, Rahim Soleimani-Jelodar2, Somayeh Jalilvand1
1Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Retrovirology
|February 3, 2026
概括
人体内源逆转录病毒 (HERVs) 在宫癌 (CC) 进展中表达的增加. 这项研究将HERV-E的表达与子宫病变联系起来,这表明在HPV感染的同时,它可能在CC发育中发挥作用.
科学领域:
- 在瘤学瘤学.
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 子宫癌 (CC) 与人类乳头瘤病毒 (HPV) 感染有着密切的联系.
- 其他因素,包括人类内源逆转录病毒 (HERVs),可能会影响CC的发展.
- HERVs是复原病毒感染的残留物,集成到宿主基因组中,可能在疾病中发挥作用.
研究的目的:
- 为了研究宫活检中HERV-E env转录的表达状态.
- 确定HERV-E表达和宫癌进展之间的关联.
- 探索HERV-E基因组,HPV基因型和患者年龄之间的相关性.
主要方法:
- 使用实时PCR对HERV-E env,HPV 16/18 E6/E7和GAPDH基因表达的分析.
- 在111次宫活检 (癌症,癌前,正常) 中对HERV-E对mRNA相对水平的量化.
- 接收器运行特征 (ROC) 曲线分析以评估诊断潜力.
主要成果:
- 与癌前病变相比,在宫癌中观察到HERV-E env表达显著增加 (1.5倍变化,P=0.031).
- 在HPV 16/18感染患者中,癌症与癌前群体中发现了HERV-E env mRNA水平的升高.
- ROC分析证实了癌前病变和癌症病变之间的ENV表达的显著差异 (P=0.015总体,P=0.023对于HPV16/18+).
- 在HPV16感染患者中,在HERV-E env mRNA和HPV 16 E7上蛋白 (R=0.34,P=0.016) 和年龄 (R=0.35,P=0.016) 之间发现了正相关性.
结论:
- 在宫病变的进展过程中,HERV-E env被积极转录,这表明与宫癌的发展有潜在的关联.
- 这些发现表明HERV-E env在CC病变发生过程中可能发挥作用,可能与HPV相互作用.
- 对HERV-E env和HPV 16 E7相互作用的进一步研究可能会揭示HPV相关癌症的常见途径.
相关概念视频
Retroviruses
15.0K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
15.0K
Mechanisms of Retrovirus-induced Cancers
7.0K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
7.0K
Retrovirus Life Cycles
49.6K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
49.6K
Chromatin Position Affects Gene Expression
24.8K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.8K
Transcription Factors
82.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.7K
Master Transcription Regulators
7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K


