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基于CRISPR/Cas12的新型检测方法,用于快速准确地检测驴肉

Mohammed Abd Alabbas Molaghi¹, Wisam Raheem Atiyah¹, Asseel Abdul Ridha Saeed¹

¹Department of Veterinary Public Health, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq.

Open veterinary journal

|February 3, 2026

概括

一种新的脱氧核糖核酸 (DNA) 测定方法可以在40分钟内准确检测驴肉. 这种快速,具体和敏感的方法提供了一种经济有效的解决方案,可以在没有专门设备的情况下识别肉类欺诈行为.

科学领域:

食品安全和真实性测试食品安全和真实性测试
分子诊断学分子诊断学
畜牧业商品验证验证畜牧业商品验证

背景情况:

准确识别动物产品对于畜牧产品的真实性至关重要.
基于核酸的方法,如聚合酶链反应 (PCR) 检测肉类欺诈,但成本昂贵,需要专业知识.
需要可访问,快速和精确的检测方法来检测来自动物的产品.

研究的目的:

开发一种基于脱氧核糖核酸 (DNA) 的精确和快速测定方法来检测驴肉.
创建一个具有成本效益和用户友好的肉类真实性的诊断工具.

主要方法:

开发了一种新的测定方法,将集群定期间隔的短Palindromic重复 (CRISPR) / Cas12,重组酶聚合酶放大 (RPA) 和侧向流装置结合起来.
针对线粒体D环基因内的保存序列,用于特定物种的识别.
基于灵敏度,特异性和周转时间的评估测试性能.

主要成果:

该试验在检测驴肉时显示出100%的特异性,没有来自其他物种的交叉反应或假阳性.
实现了高灵敏度,能够检测到输入DNA的仅为5 ng.
在没有专用设备的情况下,在40分钟内提供了包括DNA提取在内的完整结果.

结论:

开发的试验是一种可行的选择,可以快速准确地检测驴肉.
需要进一步的研究来优化试验,以便在不需要DNA提取的情况下直接检测.

关键词:

在CRISPR/Cas12中使用.驴肉驴肉驴肉是一种肉类.在 RPA RPA 中使用.

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