循环中的miRNAs与AFP相结合,可以提高HBV相关肝细胞癌的诊断准确性
Ngo Dieu Hoa1, Dao Phuong Giang2, Nguyen Thi Thu Hien3
1Hanoi Medical University, Hanoi, Viet Nam.
Biochemistry and biophysics reports
|February 3, 2026
概括
通过将microRNAs (miRNAs) 与alpha-fetoprotein (AFP) 结合起来,可以改善肝细胞癌 (HCC) 的早期检测. 这些新型生物标志物提高了与HBV相关的HCC的诊断准确性,有助于早期查工作.
科学领域:
- 肝病学和瘤学研究.
- 生物标志物的发现和验证.
- 病毒性肝炎的分子诊断.
背景情况:
- 肝细胞癌 (HCC) 的早期检测受到α-fetoprotein (AFP) 的低精度的阻碍.
- 循环中的microRNAs (miRNAs) 显示出作为癌症检测的稳定,非侵入性生物标志物的承诺.
- 乙型肝炎病毒 (HBV) 感染是全球HCC的主要原因,需要改进的诊断工具.
研究的目的:
- 为了评估血清miR-21-5p,miR-34a-5p和miR-30d-3p的诊断性能.
- 评估这些miRNAs与AFP对于与HBV相关的HCC的联合诊断价值.
- 为了区分与HBV相关的HCC与慢性乙型肝炎 (CHB) 和肝硬化 (LC) 在越南队列中.
主要方法:
- 招募450名参与者:150名HCC,150名LC,150名HBV感染的CHB患者.
- 使用定量逆转录聚合酶链反应 (RT-qPCR) 量化血清miRNA表达的量化.
- 使用接收器操作特征 (ROC) 曲线分析评估诊断准确性.
主要成果:
- 血清miR-21-5p,miR-34a-5p和miR-30d-3p在HCC患者中显著上调 (p < 0.001).
- 个别的miRNAs显示了中等的诊断性能 (AUC 0.74-0.92).
- 将三个miRNA与AFP结合起来,在所有比较中显著提高了HCC检测的诊断准确性 (AUC 0.84-0.97).
结论:
- 血清中的miR-21-5p,miR-34a-5p和miR-30d-3p是HBV相关HCC的有希望的生物标志物.
- 这些miRNAs与AFP的结合提供了更高的诊断准确性.
- 这些生物标志物支持开发用于早期HCC查的非侵入性工具.
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