通过限制发育基因表达和维持BRG1表达,CHD4确保了牛早期胚胎发生
Shuang Li1,2, Lieying Xiao2, Yan Shi2
1Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, Xinjiang, China.
概括
染色体重塑剂CHD4对于牛的早期发育至关重要. 胚胎中CHD4的枯竭会阻止发育并改变基因表达,强调其在胚胎发生过程中的重要作用.
科学领域:
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 依赖ATP的染色质重塑蛋白调节基因表达和染色体功能.
- 在哺乳动物胚胎发生过程中CHD4的作用尚不完全理解,尽管它已知在小鼠体形成和植入过程中的功能.
研究的目的:
- 为了研究CHD4在早期牛胚胎发生中的特定物种功能.
- 阐明CHD4影响牛胚胎发育的分子机制.
主要方法:
- 牛胚胎中的CHD4消耗.
- 对16细胞胚胎进行转录组分析 (RNA-seq).
- 使用测序 (ATAC-seq) 检测转化酶可访问的染色体.
- 免疫光用于基因素修饰.
主要成果:
- 由于CHD4的枯竭,导致16-32细胞阶段的发育停止,并且无法形成芽细胞.
- 失去CHD4导致652个基因的差异表达,包括许多发育基因.
- 改变的基因表达与染色质可访问性和基因组修饰的变化相关 (H3K27ac,H3K9me3).
- 由于CHD4的减少,多发性皮肤标记物氨酸的表达和全球色氨酸的可访问性增加.
- 在缺乏CHD4的胚胎中,BRG1蛋白水平显著降低.
结论:
- CHD4对于防止发育停滞和确保牛早期发育过程中适当的基因表达至关重要.
- CHD4限制发育基因表达并维持BRG1水平,促进16至32细胞阶段的过渡.
- 这些发现揭示了CHD4在牛胚胎发生过程中的关键性,物种特异性的作用.
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