在体外 (in vitro) 中,MSC通过Uvence工作流程处理的冷保存的脂肪干细胞的表征
Ahsan A Khan1, Olivier D Amar1, Sumila Rathnayaka M1
1Health Medical Bank Ltd (Uvence), Sandwich, United Kingdom.
Tissue engineering. Part C, Methods
|February 3, 2026
概括
使用Uvence工作流程进行脂肪组织加工,显示出高解后体血管分数 (SVF) 活力,超过监管标准. 这种经过验证的冷保存方法为再生医学应用提供了一个稳定且兼容的平台.
科学领域:
- 再生医学是一种再生医学.
- 脂肪组织工程 脂肪组织工程
- 细胞疗法细胞疗法
背景情况:
- 脂肪组织是再生疗法的丰富 stromal 细胞来源.
- 目前的流体血管分量 (SVF) 和纳米脂肪处理系统缺乏经过验证的冷保存和监管监督.
- 来自脂肪组织的介酶体 stromal 细胞由于可访问性和免疫调节性质而具有吸引力.
研究的目的:
- 评估Uvence专有的脂肪组织加工工作流程,重点关注冷保存和解后的生存能力.
- 评估从冷保存的脂肪组织中获得的树皮细胞的质量和特征.
- 解决SVF和纳米脂肪应用的验证结解性能上的差距.
主要方法:
- 使用Uvence集成的洗,冷保存,解和乳化工作流程在受监管的实验室中进行脂肪组织加工.
- 环境监测与良好制造实践 (GMP) 附件1和独立质量控制 (QC) 测试保持一致.
- 解后细胞活力的评估,流动细胞计量,形态学和扩展性树皮细胞的三线分化.
主要成果:
- 冷保存的脂肪组织样本的平均解后生存率约为91%,超过IFATS/ISCT设定的70%的值.
- 乌文斯在解后的生存能力与酶和机械加工平台报告的新鲜结果相当或优于新鲜结果.
- 流细胞计证实了表达CD73,CD90和CD105的 stromal 细胞,最小的CD34/CD45,符合中介细胞 stromal 细胞的 ISCT 标准.
结论:
- 乌文斯工作流提供了一种经过验证和符合标准的方法,用于处理具有强大的冷保存的脂肪组织.
- 该过程确保了高解后细胞活力,解决了再生医学的关键需求.
- 实验室表征证明了Uvence处理的脂肪衍生的树皮细胞在研究应用中的潜力.
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