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在植物性肉类,奶酪和鱼类替代品中的真菌毒素发生和风险评估,基于适应的UHPLC-MS/MS多方法
Sarah Schneidemann-Bostelmann1, Yannick Otting1, Fabian Dick1
1Chair of Analytical Food Chemistry, Technical University of Munich, Freising, Germany.
Mycotoxin research
|February 3, 2026
概括
一种新的UHPLC-MS/MS方法量化了植物性食品中的15种真菌毒素. 替代菌毒素很常见,特别是在seitan中,对幼儿构成潜在的风险,尽管在监管范围内.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 动物产品的植物替代品市场不断增长.
- 需要强大的分析方法来确保新型食品类别的食品安全.
- 菌毒素污染是食品中的一个问题,需要特定的检测方法.
研究的目的:
- 开发和验证一种超高性能液体色谱-并联质谱法 (UHPLC-MS/MS) 方法.
- 同时量化来自Fusarium,Alternaria和Aspergillus物种的15种真菌毒素.
- 评估植物性肉类,奶酪和鱼类替代品中的真菌毒素污染及其相关风险.
主要方法:
- 使用UHPLC-MS/MS进行15种真菌毒素的同时定量.
- 使用QuEChERS进行样本提取和分散固相提取 (dSPE) 进行清理.
- 通过稳定同位素稀释分析 (SIDA) 实现量化.
主要成果:
- 方法证明了从0.01μg/kg到14.1μg/kg的敏感检测极限 (LOD) 和从0.02μg/kg到41.9μg/kg的量化极限 (LOQ).
- 对32种植物性产品的分析揭示了与Alternaria毒素 (alternariol单甲基乙烯和亚酸) 的普遍污染.
- 基于赛坦的产品显示了最高的污染水平;32种产品中有27种含有至少一种真菌毒素.
结论:
- 开发的UHPLC-MS/MS方法对于植物替代品中的真菌毒素分析是有效的.
- 阿尔特纳里亚毒素构成潜在的风险,特别是对于幼儿,需要进一步监测.
- 检测到的真菌毒素水平没有超过监管限制,突出了植物替代品的特定食品安全法规中的差距.
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