一个因素整合转录和压制的表面抗原基因在非洲试生体的基因
María Agustina Berazategui1, Ione Goodwin1, Lianne I M Lansink2
1Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom.
概括
ESBX是一种新型蛋白质,用于协调Trypanosoma brucei中的基因表达. 它确保只有一个变异的表面糖蛋白 (VSG) 得到表达,这对于寄生虫免疫逃避至关重要.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 虫虫通过变异性表面糖蛋白 (VSG) 切换用于免疫逃避而采用抗原变异.
- 单基VSG表达是由RNA聚合酶I (RNA Pol I) 在表达位体 (ESB) 内的血液表达位点 (BESs) 调节的.
- 集成活跃BES转录和非活跃BES沉默的机制仍然不清楚.
研究的目的:
- 为了确定协调Trypanosoma brucei中BES转录和沉默的因素.
- 阐明ESBX在调节单基VSG表达中的作用.
主要方法:
- 对于ESBX knockdown 的RNA干扰 (RNAi).
- 过度表达ESBX.
- 对RNA Pol I定位到ESB的分析.
- 转录基因分析.转录基因分析.
- VSG排除 (VEX) 现象的调查.
主要成果:
- ESBX是BSF特有的ESB蛋白,对于协调BES转录至关重要.
- ESBX knockdown 破坏了 RNA Pol I 的定位,减少了活跃的 BES 转录,并消除了不活跃的 BES.
- 过度表达ESBX导致不活跃BES的过程性转录,而不会影响活跃BES.
- 通过VEX现象,ESBX将主动BES激活与非主动BES静音联系在一起.
结论:
- 在Trypanosoma brucei中,ESBX是单基VSG表达的关键调节者.
- 了解ESBX的功能,可以了解寄生虫的免疫逃避策略.
- 本研究提出了建立和维护单基基表达的模型.
相关概念视频
02:16Transcription Factors
82.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.8K
02:35Transcription Elongation Factors
14.0K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
14.0K
02:35Transcription Elongation Factors
4.8K
4.8K
01:30General Transcription Factors
7.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.1K
01:52Eukaryotic Transcription Inhibitors
11.0K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.0K
01:15Line, Surface, and Volume Integrals
4.3K
A line integral for a vector field is defined as the integral of the dot product of a vector function with an infinitesimal displacement vector along a prescribed path. If the prescribed path is closed, the integrals reduce to a closed-line integral. The closed-contour integral of the vector field is referred to in terms of the circulation of the vector field around the closed path. A vector with zero circulation around every closed path is called a conservative field, while one with non-zero...
4.3K