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增长速度影响了乳球球菌的风味形成,这与代谢蛋白质水平无关
Avis Dwi Wahyu Nugroho1, Berdien van Olst2, Douwe Molenaar3
1NIZO Food Research, Kernhemseweg 2, 6718 ZB, Ede, the Netherlands; Host-Microbe Interactomics Group, Wageningen University & Research, De Elst 1, 6708 WD, Wageningen, the Netherlands.
International journal of food microbiology
|February 3, 2026
概括
乳制品初级品种的增长速度较慢,如乳球球菌 (L. cremoris) 显著降低了关键风味化合物的生产. 这表明,优化快速生长可能会对发酵乳制品的感官质量产生负面影响.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
背景情况:
- 乳制品初始培养对发酵产品质量至关重要.
- 优化快速酸化是常见的,但其对味道的影响还不太清楚.
- 二次代谢物,如风味化合物,对于乳制品的特性至关重要.
研究的目的:
- 调查乳球球菌 (Lactococcus cremoris) 的生长率与二次代谢物 (风味化合物) 的形成之间的关系.
- 为了确定调节生长率是否会影响与代谢和风味生产相关的蛋白质表达.
- 评估前培养生长率对一种奶酪模型系统中挥发性化合物形成的影响.
主要方法:
- 在定义的介质和牛奶中使用部分翻译抑制和温度变化调节的*L. cremoris*NCDO712生长速度.
- 通过完全转化抑制保存蛋白质,并在定义的介质或奶酪模型中14天内化细胞.
- 分析了挥发性化合物形成和蛋白质组概况,以确定蛋白质集群 (与核糖体相关,与代谢相关,核心).
主要成果:
- 在定义的介质中,部分翻译抑制增加了与核糖体相关的蛋白质和降低了氨基酸代谢蛋白质,影响了3-甲基布坦的形成.
- 温度驱动的生长速度变化和牛奶中的部分翻译抑制对与核糖体相关的和代谢性蛋白质部分的影响很小.
- 较慢的培养前生长显著降低了关键风味化合物的产量,高达30倍,独立于酶水平.
结论:
- *L.cremoris*的快速生长并不一定会影响代谢蛋白质水平.
- 培养前的生长速度显著影响乳制品应用中的二次代谢物 (风味) 生产.
- 了解生长动力学和代谢输出之间的相互作用对于优化乳制品初始培养对于酸化和风味发展至关重要.
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