冷藏,温暖的结果:在经过长时间的冷保存后,造血干细胞的生存能力
Riana Cockeran1, Lerato Makuoane1, Tanya Glatt2
1South African National Blood Service, Johannesburg, South Africa.
概括
造血原始干细胞 (HPSCs) 在长期冷保存后保持高活力,长达13年. 这证实了干细胞储存协议在临床应用中的稳定性.
科学领域:
- 血液学 血液学 血液学
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
背景情况:
- 造血原生干细胞 (HPSCs) 在化疗后重建血细胞生产至关重要.
- 对HPSCs的冷保存为血液性恶性瘤的干细胞移植时间提供了灵活性.
- 标准化的协议对于确保冷保存的HPSCs的可行性至关重要.
研究的目的:
- 评估根据特定协议处理和储存的造血原生干细胞 (HPSC) 的长期生存能力.
- 评估延长储存时间对HPSC可行性的影响.
- 为了验证冷保存对长期干细胞储存的有效性.
主要方法:
- 对50个冷保存的HPSC产品进行分析,存储时间为12-156个月.
- 使用流细胞计和修改的ISHAGE协议进行解后评估.
- 基于CD34+和CD45+细胞计数和7-AAD染色的活力评估.
主要成果:
- 所有冷保存的HPSC产品都达到预定义的可行性值.
- 平均CD34+细胞活力为98.96%和CD45+细胞活力为66.58%.
- 存储时间最长的样本 (13年) 显示活力高 (99% CD34+, 59% CD45+),存储时间和活力之间没有相关性.
结论:
- 造血原生干细胞 (HPSCs) 在冷保存的13年内保持高活力.
- 采用的冷保存协议是强大的,并支持长期的干细胞储存.
- 这些发现加强了使用冷保存的HPSC用于移植的临床可行性.
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