在类植物中通过SHI/STY家族的转录因子进行棘特异化
Qingyou Zheng1, Shiyun Zhou1, Vivian F Irish2
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Hubei Hongshan Laboratory, Wuhan 430070, Hubei, China.
Current biology : CB
|February 3, 2026
概括
棘标识3 (TI3),一种转录因子,通过激活TI1和TI2基因,控制果中的棘发育. 被 CsCENTRORADIALIS (CsCEN) 压制,TI3 操纵为培育无刺植物提供了一个新的策略.
科学领域:
- 植物生物学 植物生物学
- 发育遗传学的发展遗传学.
- 进化植物学是进化的植物学.
背景情况:
- 棘是植物的防御结构,独立地进化了多次.
- 棘标识1 (TI1) 和TI2转录因子是已知的调节者在的棘发育.
- 控制棘发展的上游监管机制在很大程度上是未知的.
研究的目的:
- 为了确定果中棘发育的新型调节剂.
- 阐明在棘形成和调节的基础上的分子机制.
- 为了探索培育无刺品种的潜力.
主要方法:
- 比较性转录组学以确定在棘中差异表达的基因.
- 促进体分析以确定转录因子结合部位.
- 使用CRISPR-Cas9基因编辑来评估基因功能.
- 对相关物种同源基因的分析.
主要成果:
- 鉴定了TI3,一个SHORT INTERNODES/STYLISH (SHI/STY) 家族的转录因子,具体表达为棘.
- TI3通过与CTAG核心促进元件结合,直接激活TI1和TI2的表达,从而促进干细胞的停止.
- 由于CRISPR-Cas9对TI3的破坏,导致了棘转为分支的转换.
- CsCENTRORADIALIS (CsCEN) 抑制TI3的表达,维持干细胞活动以促进分支的发展.
- 在CsCEN中发生的突变导致了分支到棘的转换,双重突变显示了ti3表型.
结论:
- TI3是一种新的,关键调节者,在果中对棘发育和美里系统的认同.
- CsCEN-TI3监管模块控制了棘和树枝发展之间的切换.
- TI3同类物体在 Rutaceae 种类的棘发育中表现得保留.
- 准TI3为开发无棘植物品种提供了一个有前途的战略.
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