通过NIR-II光和热对比增强多重侧流免疫试验的灵敏度
Yi-Chi Luo1, Yung-Chun Hsieh2, Chun-Yang Huang1
1Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.
Analytical chemistry
|February 3, 2026
概括
这项研究引入了一种新型的多式侧流检测 (LFA) 平台,可提高灵敏度,并使用色度,光热和NIR-II光读数进行癌症生物标志物的多重检测,以改善护理点诊断.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 分析化学 分析化学
背景情况:
- 侧流检测 (LFA) 对于临床诊断至关重要,但往往缺乏实验室方法的敏感性和特异性.
- 现有的LFA在高精度同时检测多个生物标志物方面存在局限性.
研究的目的:
- 开发一个多模式的LFA平台,集成色度,光热和第二近红外窗 (NIR-II) 光读数.
- 提高灵敏度并使癌症生物标志物的多重检测成为可能,以改善POC诊断.
主要方法:
- 金纳米棒与NIR-II发射聚合物点相结合,创建了可增强等离子体光和光热信号的探针.
- 碳水化合物抗原15-3 (CA15-3) 和癌胚抗原 (CEA) 被用作乳腺癌和一般癌症查的模型生物标志物.
- 该平台使用临床血清样本进行了验证,并与标准电化学发光免疫测试进行了比较.
主要成果:
- 多模式LFA使用温度计和NIR-II光模式实现了CA15-3 (0.40 U/mL) 和CEA (0.096 ng/mL) 的敏感检测.
- 在单一条带上同时检测两种生物标志物显示出最小的交叉反应性.
- 临床血清样本验证表明与已建立的诊断方法有很强的相关性.
结论:
- 开发的便携式低成本平台协调了NIR-II光和光热读数,用于敏感,选择性和多重的POC癌症生物标志物检测.
- 这种通用信号放大概念为下一代LFA提供了有希望的进步.
- 该平台可以适应检测其他生物标志物,扩大其诊断应用.
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