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相关概念视频

Termination of Translation01:44

Termination of Translation

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The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
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Termination of Translation01:44

Termination of Translation

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What is Variation?01:14

What is Variation?

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Apart from the measures of central tendency, distribution, outliers, and the changing characteristics of data with time, an important characteristic of any data set is its variation or spread. In some data sets, the data values are concentrated closely near the mean; in others, the data values are more widely spread out from the mean.
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Variation01:19

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An important characteristic of any set of data is the variation in the data. In some data sets, the data values are concentrated closely near the mean; in other data sets, the data values are more widely spread out from the mean. The most common measure of variation, or spread, is the standard deviation, which is the square root of variance.
When independent and dependent variables are plotted on a scatter plot, the slope of a line is a value that describes the rate of change between the two...
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Variation of Atmospheric Pressure01:18

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Change in atmospheric pressure with height is particularly interesting. The decrease in atmospheric pressure with increasing altitude is due to the decreasing gravitational force per unit area as we move away from the surface of the earth.
Assuming the air temperature is constant at a given altitude and that the ideal gas law of thermodynamics describes the atmosphere to a good approximation, one can find the variation of atmospheric pressure with height.
Let p(y) be the atmospheric pressure at...
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相关实验视频

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The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
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蛋白质C端变异会影响蛋白质静止.

Ching-Yu Chu1,2, Shu-Yu Hsu1,2, Chi-Wei Yeh1

  • 1Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.

Nature communications
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概括

改变的蛋白C-终端可以令人惊地稳定或破坏蛋白质的稳定性,影响蛋白质组的多样性. 这项研究揭示了与疾病相关的C端变化会影响蛋白质稳定性,超出质量控制范围.

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科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 蛋白质C端因错误或编程调节而表现出变异性,有助于蛋白质组的多样性.
  • 这些C端变异对蛋白质稳定性和功能的确切影响尚未完全理解.
  • 异常的C末端通常与蛋白质降解有关,但这种联系可能不是普遍的.

研究的目的:

  • 为了研究C-终端变异如何由不间断突变,替代拼接和转化读透产生,影响蛋白质半衰期.
  • 为了确定与疾病相关的C端变化是否会影响关键蛋白质如coproteins和瘤抑制剂的稳定性.
  • 识别区分错误的C-终端与野生类型的特征,并了解它们的向由ubiquitin连接酶网络.

主要方法:

  • 分析来自不间断突变,替代拼接和转化阅读的C端变异.
  • 评估蛋白质半衰期对C端修饰的反应.
  • 鉴定特定的C端特征及其与无素酶网络的相互作用.

主要成果:

  • 与之前的假设相反,错误的C末端可以稳定或破坏蛋白质的稳定.
  • 发现与疾病相关的不间断突变改变了许多coproteins和瘤抑制剂的稳定性.
  • 观察到C端变异通常会影响正规蛋白质的稳定性,这表明蛋白质质量控制之外的作用.
  • 水性C-终端被确定为复杂的ubiquitin结合酶网络的目标.

结论:

  • C端变异是影响蛋白质稳定性和蛋白质组多样性的重要因素,其影响超出了蛋白质质量控制范围.
  • 与疾病相关的C端变化可能会影响关键癌症相关蛋白质的稳定性.
  • 无素酶网络在向特定的C端特征方面发挥着作用,突出了蛋白质降解的调节机制.