功能性食补充剂的HPLC-DAD分析,其次是液体-液体微提取辅助FTIR识别IR活性成分的IR活性成分
Denis Pavelek1, Massoud Kaykhaii2,3, Josef Jampilek1
1Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University Bratislava, Mlynská Dolina CH-2, Ilkovičova 6, Bratislava, 842 15, Slovakia.
Scientific reports
|February 3, 2026
概括
一种新的液体-液体微提取 (LLME) 辅助的里埃变换红外线 (FTIR) 方法改善了补充剂中活性成分的识别. 这种技术克服了在高性能液态染色学 (HPLC) -FTIR分析中的溶剂干扰,以更好地控制质量.
科学领域:
- 分析化学 分析化学
- 频谱学是一种光谱学.
- 染色体学 染色体学 是一种染色体学.
背景情况:
- 准确识别食补充剂和功能饮料中的活性成分对于消费者安全和产品质量至关重要.
- 福里埃变换红外光谱法 (FTIR) 与高性能液态色谱法 (HPLC) 结合,提供了分子洞察力,但受到溶剂干扰.
- 补充剂和饮料中的复杂矩阵对直接光谱分析构成挑战.
研究的目的:
- 开发和验证液体-液体微提取 (LLME) 辅助的HPLC-FTIR方法,以有效隔离和识别生物活性化合物.
- 在复杂样品的FTIR分析中克服溶剂和盐干扰的局限性.
- 为补充剂和功能饮料的常规质量控制和监管测试提供实用解决方案.
主要方法:
- 使用高性能液态色谱 (HPLC) 来分离分支链氨基酸饮料的成分.
- 使用红外透明,非极性溶剂的液体-液体微提取 (LLME) 应用于HPLC分量,以去除移动相残留物和盐.
- 优化的LLME参数,溶剂组成和缓冲效应与FTIR分析的常规溶剂蒸发进行了比较.
主要成果:
- 在LLME辅助的HPLC-FTIR方法成功消除了溶剂和盐的干扰,使得清晰的FTIR光谱.
- 关键化合物包括酸,L-亚斯科布酸,氨酸,氨酸,异氨酸和咖啡因被准确地确定.
- 与传统的溶剂蒸发技术相比,开发的方法显示出更高的性能.
结论:
- 通过LLME辅助的HPLC-FTIR是一种强大而实用的方法,用于分析补充剂和饮料中的复杂矩阵.
- 这种方法为基于红外线的活性成分的识别提供了可靠的途径,提高了产品质量评估.
- 该技术与标准实验室设备和染色学系统兼容,可在质量控制和监管环境中进行常规应用.
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