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通过水Ceratopteris richardii的空间转录组分析揭示了核糖体蛋白基因表达的空间调节

Jin Han¹, Gui-Sheng Li¹

¹Laboratory of Plant Resource Conservation and Utilization, Jishou University, Jishou, China.

Frontiers in plant science

|February 4, 2026

概括

空间转录学揭示了在水杂虫的发展过程中明显的细胞群. 意想不到的是,核糖体蛋白质基因在整个组织中表达不同,为植物生长提供了新的见解.

科学领域:

植物发育生物学植物发育生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

空间转录学为复杂的生物系统提供了新的见解.
了解植物发育需要详细的空间基因表达数据.

研究的目的:

使用空间转录学绘制水 (Ceratopteris richardii) 胞体发育过程中的基因表达模式.
识别与不同发育组织相关的标记基因和生物过程.

主要方法:

在Ceratopteris richardii sporophytes.应用空间转录学.
识别和分析不同的空间表达集群.
对已识别的标记基因进行功能丰富分析.
在现场杂交用于验证基因表达模式.

主要成果:

确定了11个不同的空间集群,对应于各个叶子阶段,原始,射出角髓 (SAM),茎,血管和/三合体.
转录因子家族 (ARF,GRF,AP2,bZIP) 被确定为标记基因.
功能性丰富揭示了先进叶子中的质体相关功能和线粒体组织中的DNA螺旋酶活性.
突如其来地观察到,各组织中的核糖体蛋白 (RP) 基因的普遍和差异性表达,并呈现出不同的模式.

结论:

空间转录组学对于剖析植物发育是有效的.
RP基因的差异表达表明植物组织中具有复杂的调节作用.
这项研究提供了一个高分辨率的空间基因表达地图,用于Ceratopteris胞体的发展.

关键词:

科拉托普特里斯·里查尔迪 (Ceratopteris richardii) 是一种类动物.在现场混合化.叶子普里莫迪亚 (Primordia) 是一个核糖体蛋白质基因核糖体蛋白质基因空间转录组空间转录组血管系统血管系统

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