逃脱 格拉姆阴性细菌在非生物表面的干燥后逃脱基于培养的检测
Daniela Visaggio1,2,3, Massimiliano Lucidi1,2, Cinzia Spagnoli1
1Department of Science, Roma Tre University, Rome, Italy.
Microbiology spectrum
|February 4, 2026
概括
医院获得的ESKAPE病原体可以在表面进入可生存但不可培养 (VBNC) 状态,从而逃避检测. 一个简单的复苏步骤显著改善了这些传染性阴性细菌的恢复.
科学领域:
- 微生物学 微生物学
- 预防和控制感染的预防和控制.
- 环境健康 环境健康
背景情况:
- 逃生病原体是导致医院感染和多药耐药性的主要原因.
- 医院表面是病原体传播的水库,但目前的检测方法错过了可生存但不能培养 (VBNC) 细菌.
- VBNC细菌在代谢上活跃,具有潜在的传染性,但在标准培养物中无法生长.
研究的目的:
- 调查ESKAPE病原体是否在医院表面干燥后进入VBNC状态.
- 为了确定VBNC ESKAPE病原体是否可以复活和恢复.
- 为了比较基于复苏的方法与标准生物污染控制的疗效.
主要方法:
- 在各种非生物表面 (玻璃,塑料,棉花,) 上一周干燥ESKAPE病原体.
- 在无碳缓冲中复苏干燥的细菌.
- 基于培养的检测和与标准生物污染控制方法的比较 (EN 17141:2020).
主要成果:
- 所有ESKAPE病原体在干燥后的培养能力降低,因物种和表面而异.
- 格拉姆阳性ESKAPE物种在复苏后没有恢复培养能力.
- 格拉姆阴性ESKAPE物种恢复到可种植状态,证实了VBNC过渡.
- 与标准方法相比,在培养前的复苏显著增加了格拉姆阴性VBNC细菌的恢复.
结论:
- 临床上相关的グラム阴性ESKAPE病原体可以在VBNC状态下在医院表面存在,导致污染的低估.
- 目前基于培养的环境监测方法不足以检测VBNC病原体.
- 整合一个复苏步骤可以提高检测灵敏度,这对于准确地评估表面污染和预防感染至关重要.
相关概念视频
Gram-negative Bacterial Protein Secretion Systems
952
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
952
Escape Velocity
8.4K
The escape velocity of an object is defined as the minimum initial velocity that it requires to escape the surface of another object to which it is gravitationally bound and never to return. For example, what would be the minimum velocity at which a satellite should be launched from the Earth's surface such that it just escapes the Earth's gravitational field?
To calculate the escape velocity, it is assumed that no energy is lost to any frictional forces. In practice, a satellite...
To calculate the escape velocity, it is assumed that no energy is lost to any frictional forces. In practice, a satellite...
8.4K
Escape Velocities of Gases
1.4K
To escape the Earth's gravity, an object near the top of the atmosphere at an altitude of 100 km must travel away from Earth at 11.1 km/s. This speed is called the escape velocity. The temperature at which gas molecules attain the rms speed, which is equal to the escape velocity, can be estimated by using the equation for the average kinetic energy of the gas molecules. According to the kinetic theory of gas, the average kinetic energy of the gas molecules is proportional to its...
1.4K
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
677
Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
677
Negative Regulator Molecules
38.5K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.5K
Positive, Negative, and Zero Work
22.2K
Work is done on an object when energy is transferred to the object. In other words, work is done when a force acts on a body that undergoes a displacement from one position to another. By definition, the work done by a force is the integral of the force with respect to the displacement along its path. Forces can vary as a function of position, and displacements can occur along various paths between two points. The magnitude of a force multiplied by the cosine of the angle that the force makes...
22.2K


