史蒂维oside通过激活AMPK/NRF2信号通路来缓解CONA诱导的肝损伤
Zhe Zhang1, Yidan Wang1, Jinping Hu1
1Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, China.
Journal of agricultural and food chemistry
|February 4, 2026
概括
石酸是一种天然的甜味剂,通过激活AMPK/NRF2通路来保护肝脏免受损伤. 这项研究证明了其在预防和治疗肝损伤方面的治疗潜力.
科学领域:
- 药理学和毒理学 药理学和毒理学
- 肝病学 肝病学是一种肝病学.
- 自然产品化学 自然产品化学
背景情况:
- Стевиозид,来自*Stevia rebaudiana*,是FDA批准的食补充剂,具有已知的抗炎,抗氧化和抗性质.
- 对于康卡纳瓦林A (Con A) 诱导的肝损伤,史蒂维oside的治疗潜力尚不清楚.
- 了解化在肝损伤中的机制,可能会揭示新的治疗策略.
研究的目的:
- 在小鼠模型中研究石化对康卡纳瓦林A (Con A) 诱导的肝损伤的保护作用.
- 为了阐明底层的分子机制,负责史蒂维oside的肝脏保护作用.
- 在肝损伤的背景下,识别石化的潜在分子标.
主要方法:
- 采用了一种康卡纳瓦林A (Con A) 诱导的小鼠肝损伤模型.
- 使用血清生物化学和组织病理学来评估肝损伤.
- 肝转录学,分子对接,CETSA和DARTS测试被用于确定关键路径和目标.
- 对RAW264.7细胞进行了体外研究,并在体内进行了关键途径的遗传/药理抑制.
主要成果:
- 稳酸治疗剂量依赖性降低了血清肝损伤标志物,并改善了他的病理性肝损伤.
- 减弱的Stevioside-Con A诱导的肝炎和肝细胞亡,缓解氧化应激.
- 转录组分析表明,氧化还原酶活性通路的丰富.
- 分子对接,CETSA和DARTS测定确定AMPK作为一个关键的目标.
- 石酸激活AMPK/NRF2信号通路,由体外和体内实验证实.
结论:
- 石酸具有显著的肝脏保护作用,可以防止Con A诱导的肝损伤.
- 该机制涉及通过与AMPK直接相互作用激活AMPK/NRF2信号通路.
- 石酸显示出作为预防和治疗肝脏疾病的治疗剂的潜力.
- 这项研究为使用石化的新型肝损伤治疗提供了基础.
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