在糖尿病病中对尿液细胞外囊衍生的非编码RNA进行系统审查:表达特征,临床相关性和诊断性能的表现
Masoomeh Hamdi1,2, Alireza Rahimizadeh1,2, Mojdeh Ahmadi1,2
1Department of Clinical Laboratory Sciences, Faculty of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Molecular biology reports
|February 4, 2026
概括
尿液细胞外囊非编码RNAs (ncRNAs) 显示为糖尿病病 (DN) 的非侵入性生物标志物具有前途. 这些分子与功能相关,可以帮助诊断DN阶段,但需要标准化.
科学领域:
- 生物化学 生物化学
- 遗传学 是一个遗传学.
- 腎臟病學 (nephrology) 是一種醫學.
背景情况:
- 糖尿病病 (DN) 是末期病的主要原因.
- 尿液细胞外囊 (uEVs) 含有非编码RNA (ncRNAs),具有作为DNA生物标志物的潜力.
- 目前的研究正在探索这些UEV衍生的ncRNAs的诊断实用性.
研究的目的:
- 系统地审查uEV衍生的ncRNAs在DNA中的表达模式.
- 评估这些ncRNA与DNA进展和相关因素的临床关联.
- 评估UEV衍生的ncRNAs用于识别DN的诊断疗效.
主要方法:
- 根据PRISMA 2020指南,在PubMed,Embase和Web of Science进行了系统的文献搜索,截至2025年3月15日.
- 包括的研究是人类观察性研究,比较uEV衍生的ncRNAs在DN患者与非DN糖尿病患者和健康对照.
- 使用ROBINS-I工具评估了偏见,并提取了ncRNA表达,临床相关性和诊断性能的数据.
主要成果:
- 包括24项研究,确定了197个尿液细胞外囊泡衍生微RNA (miRNA) 和有限数量的其他ncRNA.
- 这些miRNAs与功能标志物 (例如uACR,eGFR,血清肌素,BUN) 和白蛋白尿症阶段有显著的关联.
- 单个miRNAs (例如,miR-636,miR-34a) 显示出高诊断效率 (AUC>0.88),组合面板改善了DN分期歧视.
结论:
- 尿液细胞外囊衍生的ncRNA被验证为DNA发育的敏感指标.
- 这些ncRNAs代表了用于DN诊断和监测的有希望的非侵入性生物标志物.
- 分析程序的标准化对于提高这些发现的可复制性至关重要.
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