含有抗微生物剂的三维细菌纳米纤维素支架的抗生物膜活性
Fernanda Keil Kressin1, Isabela Aparecida Stahelin1, Gilmar da Rosa Souza1
1Department of Dentistry, Endodontics Division, University of Southern Santa Catarina, Palhoça, Santa Catarina, Brazil.
Brazilian dental journal
|February 4, 2026
概括
细菌纳米纤维素支架与阿莫西西林或克林达米辛显示早期抗生素膜活性再生内牙程序 (REP). 这些新型支架比目前的材料提供了潜在的改进,在最初的阶段减少了微生物负载.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 牙周内科医院 牙周内科医院 牙周内科医院
背景情况:
- 目前的再生性内科医疗程序 (REP) 面临现有支架的技术限制.
- 为了克服这些挑战,正在探索替代性脚手架材料.
研究的目的:
- 为了评估三维细菌纳米纤维素 (BNC) 支架的体外抗菌膜活性.
- 评估与多种生物膜相对应的含有赫西丁二甲酸盐 (CHX),阿莫西林 (AMOX) 或克林达米辛 (CLI) 的BNC支架.
主要方法:
- 使用或不使用抗微生物药物的BNC支架 (BNC/CHX,BNC/AMOX,BNC/CLI,BNC/C) 被浸入多种口腔生物膜模型中.
- 殖民地形成单位 (CFU) 在24小时,7天和15天后得到量化.
- 使用扫描电子显微镜 (SEM) 和共聚焦激光扫描显微镜 (CLSM) 可视化生物膜的形成.
主要成果:
- 与其他组相比,BNC/AMOX在24小时内显示出明显较低的CFU.
- 在SEM和CLSM中,实验小组中的微生物存在量减少,而最初的对照小组则减少.
- 虽然CFU随着时间的推移而增加,但阿莫西西林和克林达米辛在早期表现出优异的抗菌膜活性.
结论:
- 结合1%的阿莫西西林或1%的克林达米辛的BNC支架显示出有前途的早期抗生素膜疗效.
- 这些带有抗菌素的BNC支架代表了再生性内牙术程序的潜在进步.
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