通过单细胞RNA测序解码人类阴茎状细胞癌的细胞异质性和恶性进展
Xiheng Hu1,2,3,4, Wensheng Shi1,5, Liang Dong2,3,4
1Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|February 4, 2026
概括
这项研究以单细胞分辨率绘制阴茎状细胞癌 (PSCC) 瘤微环境 (TME). SEMA3C被认为是预测PSCC转移和预后的有前途的生物标志物.
科学领域:
- 在瘤学瘤学.
- 生殖尿道恶性瘤
- 单细胞分析 单细胞分析
背景情况:
- 阴茎状细胞癌 (PSCC) 是一种罕见的癌症.
- 瘤微环境 (TME) 显著影响PSCC的进展,复发和转移.
- 了解TME异质性对于开发有效的预后指标和治疗策略至关重要.
研究的目的:
- 为了生成一个全面的PSCC TME单细胞地图.
- 识别不同的细胞群和它们在瘤进展中的作用.
- 评估SEMA3C作为PSCC淋巴结转移和预后的潜在生物标志物.
主要方法:
- 来自9个PSCC瘤样本和6个相邻的正常样本的66421个细胞的单细胞RNA测序.
- 在TME.内识别和表征不同的细胞群.
- 对细胞与细胞相互作用和参与瘤进展的分子途径的分析.
主要成果:
- 在PSCC TME中发现了9种不同的细胞群.
- 高SEMA3C的恶性细胞与上皮层-介质细胞过渡 (EMT) 有关.
- 耗尽的T细胞,高SPP1瘤相关的巨细胞 (TAMs),癌症相关的纤维细胞和POSTN+细胞周细胞都与瘤进展,血管生成和细胞外矩阵重塑有关.
- SEMA3C被确定为癌症阶段和微血管密度的生物标志物.
结论:
- 单细胞分析显示PSCC中存在显著的TME异质性.
- SEMA3C是预测淋巴结转移和PSCC预后的潜在生物标志物.
- 这些发现为开发针对PSCC TME的新型治疗策略提供了洞察力.
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