普罗西米通过TNF-α/NF-κB/ROS途径诱导肌肉缩和肌细胞融合障碍,在发育中的斑马鱼胚胎中
Chuanwen Yin1, Suwen Zeng2, Yuyang Peng3
1Jiangxi Branch of State Key Laboratory for Macromolecule Drugs and Large-scale Preparation & National Engineering Research Center of Cell Growth Factor Drugs and Protein Biologics, Center for Genetic Development and Regenerative Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, Jiangxi Province, China; School of Basic Medicine, Gannan Medical University, Ganzhou 341000, Jiangxi Province, China.
Environmental pollution (Barking, Essex : 1987)
|February 4, 2026
概括
普罗西米杀菌剂通过破坏肌肉发育和增加氧化应激,导致斑马鱼的肌肉损伤. 这项研究揭示了procymidone的存在.
科学领域:
- 环境毒理学环境毒理学
- 发育生物学是发展生物学.
- 骨肌肉生理学 骨肌肉生理学
背景情况:
- 普罗西米 (PCM) 是一种广泛使用的杀菌剂,已知具有肝毒性和内分泌干扰作用.
- 普西米的肌肉毒性尚未得到广泛的研究.
- 了解PCM对肌肉健康的影响对于环境和人类安全至关重要.
研究的目的:
- 为了研究 procymidone (PCM) 在发育中的斑马鱼的肌肉毒性.
- 阐明PCM诱导肌肉毒性的潜在分子机制.
- 评估PCM对水生生物的潜在影响.
主要方法:
- 斑马鱼胚胎在受精后72小时内暴露在PCM (1,1.25,1.5毫克/升) 的不同度下.
- 转基因斑马鱼模型被用来观察肌病性表型.
- 进行了转录组分析和基因组丰富分析 (GSEA).
- 测量了反应性氧物种 (ROS) 水平和亡.
主要成果:
- 暴露于PCM诱导了肌病性表型,包括肌肉缩和细胞融合受损.
- 观察到与肌肉发育,分化,融合和运动功能相关的基因的广泛失调.
- 在受影响的组织中检测到ROS水平升高和亡.
- 包括Toll类受体,TNF-α/NF-κB和亡在内的信号通路都受到影响.
- 抑制NF-κB部分挽救了PCM诱导的肌肉缩.
结论:
- 在发育中的斑马鱼中,普罗西米暴露会导致显著的骨肌肉毒性.
- PCM诱导的肌肉毒性与氧化应激,炎症和亡有关.
- 这些发现提供了对普罗西米对肌肉的影响的机制性见解,并突出了对水生生态系统的潜在风险.
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