器官特异性体外模型用于预测纳米材料的危险评估
Laura Maria Azzurra Camassa1, Elisabeth Elje2, Sergey Shaposhnikov3
1National Institute of Occupational Health (STAMI), 0033 Oslo, Norway.
概括
二氧化 (CeO2) 纳米粒子和银纳米线具有不同的毒性. 较小的CeO2 NMs会导致肺毒性,而较大的和银纳米线会诱导炎症反应和潜在的脑细胞损伤.
科学领域:
- 纳米毒理学研究
- 在体外毒理学.
- 生物医学工程 生物医学工程
背景情况:
- 特定器官的多细胞体外模型对于评估纳米材料 (NM) 的安全性至关重要.
- 模仿肺-血液-大脑轴需要复杂的模型来理解系统效应.
研究的目的:
- 使用先进的体外模型评估二氧化 (CeO2) NMs和银 (Ag) 纳米线的安全性和潜在毒性.
- 研究NMS对肺,血液和脑细胞的影响,评估细胞毒性,基因表达和炎症反应.
主要方法:
- 使用三培养肺模型,全血模型和星细胞-神经元共培养系统.
- 暴露在三个CeO2 NMs (不同大小) 和Ag纳米线的模型中.
- 评估的终点包括细胞毒性,基因表达,基因毒性,炎症标志物,补体激活和二次大脑毒性.
主要成果:
- 高剂量的CeO2 3.5nm NMs降低了肺细胞活力,而CeO2 50nm NMs和Ag纳米线诱导了肺和内皮细胞中的炎症标志物.
- 全血模型显示了CeO2 NMs和Ag纳米线对补体激活的差异效应.
- 来自Ag纳米线暴露的条件介质降低了脑细胞活力,而CeO2 50nm NMs则诱导了脑细胞中的炎症基因表达.
结论:
- CeO2 3.5 纳米 NMs 呈现肺毒性.
- CeO2 50nm NMs和Ag纳米线引发炎症反应,并可能通过条件介质诱导脑细胞中的细胞毒性.
- 多细胞体外模型是预测NM毒性的有效工具,沿着肺-血液-大脑轴.
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