通过Siderophore-Assisted的化物吸收和生物转化,通过丰富的Saccharomyces cerevisiae的高效生物合成
Wen-Tao Du1, Ya-Ru Wei1, Xue Zhao1
1School of Life Science and Engineering, Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province, Lanzhou University of Technology, Lanzhou 730050, China.
Bioresource technology
|February 4, 2026
概括
这项研究使用 siderophores 制造丰富酵母 (Saccharomyces cerevisiae),提高其耐受性和有机含量. 丰富的酵母具有显著的抗氧化特性,为生物可用补充剂提供了一个新的来源.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 营养科学 营养科学
背景情况:
- (Se) 对人类健康至关重要,但无机形式存在毒性风险.
- 开发生产可生物利用的有机的高效方法对于食应用至关重要.
研究的目的:
- 开发一种 siderophore 辅助的策略,以提高丰富的Saccharomyces cerevisiae 的生产.
- 研究在S. cerevisiae中无机转化为有机的生物转化.
- 为了评估丰富酵母的抗氧化潜力.
主要方法:
- 使用了来自Aspergillus sp.的 siderophore. 为了增强S. cerevisiae对化的耐受性.
- 使用先进的分析技术量化细胞内含量.
- 通过XPS和HPLC-MS确认生物转化为有机形式,主要是甲,通过XPS和HPLC-MS.
- 使用DPPH和基清除试验评估抗氧化活性.
- 进行了转录基因分析,以阐明运输机制.
主要成果:
- 赛德罗显著增加了S. cerevisiae对化的耐受性 (高达2000微克/毫升).
- 细胞内含量达到3.89 mg/g,其中97.85%转化为有机 (氨酸).
- 富含的酵母表现出强大的抗氧化活性 (DPPH 59.85%,基 97.18%).
- 转录基因数据表明, siderophore 介导的运输可能涉及 Pho91p.
结论:
- 赛德罗辅助生物生产是一种有效的策略,用于丰富的S. cerevisiae.
- 这种方法产生高度生物利用的有机,主要是甲.
- 富含的酵母是抗氧化剂补充剂的有希望的来源.
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