相关实验视频

Updated: Feb 6, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.6K

让GFP值得考虑:在精密发酵中对绝对蛋白质量定量的验证框架

Christina Peternell1, Philipp Noll1, Annette Brümmer-Rolf1

  • 1Cellular Agriculture, TUM School of Life Sciences, Technical University of Munich, Gregor-Mendel-Str. 4, Freising, 85354, Germany.

Applied microbiology and biotechnology
|February 4, 2026
PubMed
概括

本研究引入了一种高通量方法,用于使用直接光测量量量化重组绿色光蛋白 (EmGFP). 通过FDA指南验证的可访问的工作流程,可以精确量化生物过程优化中的蛋白质.

关键词:
绝对蛋白质度方法的绝对蛋白质度方法.生物过程优化优化光信号的标准化和验证.绿色光蛋白是一种绿色光蛋白.高通量蛋白质量化的量化.精密发酵精密发酵的方法

更多相关视频

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

9.7K
Light-Controlled Fermentations for Microbial Chemical and Protein Production08:37

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

4.6K

相关概念视频

Fermentation01:29

Fermentation

Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
129.4K
Absolute Value Inequalities01:23

Absolute Value Inequalities

The absolute value is a mathematical tool that represents the distance of a number from zero on the number line, regardless of its sign. In the context of inequalities, absolute value expressions help define a range of permissible values or boundaries for a variable. These inequalities are commonly used in scientific modeling and data interpretation, where variability within or beyond a certain threshold must be captured precisely.An absolute value inequality of the form ∣x∣ ≤...
338
Mean Absolute Deviation01:13

Mean Absolute Deviation

The mean absolute deviation is also a measure of the variability of data in a sample. It is the absolute value of the average difference between the data values and the mean.
Let us consider a dataset containing the number of unsold cupcakes in five shops: 10, 15, 8, 7, and 10. Initially, calculate the sample mean. Then calculate the deviation, or the difference, between each data value and the mean. Next, the absolute values of these deviations are added and divided by the sample size to...
3.4K
Reliability and Validity01:29

Reliability and Validity

Reliability and validity are two important considerations that must be made with any type of data collection. Reliability refers to the ability to consistently produce a given result. In the context of psychological research, this would mean that any instruments or tools used to collect data do so in consistent, reproducible ways.
14.0K
Microbial Fermentation01:23

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.5K
Absolute and Local Extreme Values01:22

Absolute and Local Extreme Values

The highest and lowest values of a function, relative to a reference axis, are known as extreme values. These include absolute maximum and absolute minimum values, which represent the highest and lowest points the function reaches across its entire domain. Within a restricted portion of the function, the highest and lowest values are referred to as local maximum and local minimum values, respectively.Periodic functions, such as sine and cosine, show extreme values at infinitely many points due...
79