相关实验视频
Updated: Feb 6, 2026

07:51
Pull-down of Calmodulin-binding Proteins
Published on: January 23, 2012
25.9K
卡尔莫杜林用于高纯度分离稀土元素的持续演变
Farid F Khoury1, Sameera Abeyarthna1, Mallori A Herishko1
1Department of Chemical Engineering, Columbia University, New York, New York, USA.
Angewandte Chemie (International ed. in English)
|February 5, 2026
概括
蛋白质工程推进了稀土元素 (REE) 分离. 一种新的菌体辅助连续进化方法迅速进化出一种用于高纯度REE隔离的,提供了一个可持续的替代方案.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 由于它们的特性相似,分离稀土元素 (REEs) 具有挑战性,需要复杂的工业过程.
- 基于蛋白质的方法为REE分离提供了可持续和选择性的替代方案.
- 目前用于REE分离的蛋白质工程方法受到低吞吐量的限制,限制了优化.
研究的目的:
- 开发一种高通量蛋白质工程方法,以增强REE分离.
- 开发一种来自calmodulin的,用于选择性结合和分离单个REEs.
- 为了证明菌体辅助连续进化 (PACE) 在金属结合蛋白工程中的实用性.
主要方法:
- 为PACE开发了一种兰坦化介导的蛋白质-蛋白质相互作用选择电路.
- 利用PACE快速演变一种来自calmodulin的类库来对抗REEs.
- 采用分子动力学模拟来分析进化的蛋白质中的结构变化.
主要成果:
- 在使用PACE系统的几天内确定了一个主要的进化序列.
- 分子动力学模拟显示了增强的第二离子协调和蛋白质包装.
- 进化的蛋白质表现出更好的结合亲和力和热稳定性.
- 实现了单个REEs的单阶段,无化剂,高纯度的分离.
结论:
- 开发的PACE系统显著加速了金属结合蛋白的工程.
- 进化的可以有效和选择性地分离关键的稀土元素.
- 这种高通量策略适用于为各种应用设计其他金属结合蛋白.
相关概念视频
The Evidence for Evolution
48.3K
Genetic variations accumulating within populations over generations give rise to biological evolution. Evolutionary changes can result in the formation of novel varieties and entire new species. These changes are responsible for the diverse forms of life inhabiting the planet. The evidence for evolution suggests that all living organisms descended from common ancestors.
48.3K
Periodic Classification of the Elements
59.2K
The periodic table arranges atoms based on increasing atomic number so that elements with the same chemical properties recur periodically. When their electron configurations are added to the table, a periodic recurrence of similar electron configurations in the outer shells of these elements is observed. Because they are in the outer shells of an atom, valence electrons play the most important role in chemical reactions. The outer electrons have the highest energy of the electrons in an atom...
59.2K
Convergent Evolution
33.0K
Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
33.0K
Calmodulin-dependent Signaling
6.2K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
6.2K
Elements and Compounds
105.0K
Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond.
Elements
Elements are classified as atomic or molecular based on the nature of their basic units. They are unique forms of matter with specific chemical and physical properties that cannot break down into smaller substances by ordinary chemical reactions. There...
Elements
Elements are classified as atomic or molecular based on the nature of their basic units. They are unique forms of matter with specific chemical and physical properties that cannot break down into smaller substances by ordinary chemical reactions. There...
105.0K
Gene Evolution - Fast or Slow?
8.2K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
8.2K

