全面的16S rRNA基因测序和女性生殖道微生物群的转录组分析:两个具有不同信息的分子配置文件
Alberto Sola-Leyva1,2,3, Inmaculada Pérez-Prieto1,4, Analuce Canha-Gouveia4,5,6
1Celvia CC, Tartu, Estonia.
Human reproduction open
|February 5, 2026
概括
DNA和RNA测序技术产生不同的子宫内膜微生物概况. RNA测序为病原体提供了更高的分辨率,这表明需要重新评估子宫内膜微生物组分析.
科学领域:
- 生殖健康 生殖健康
- 微生物组研究 微生物组研究
- 分子生物学分子生物学
背景情况:
- 目前对子宫内膜微生物组的理解在很大程度上依赖于基于DNA的16S rRNA基因分析.
- DNA 分析存在局限性,包括低估了微生物多样性,丰富性和功能性.
- 基于RNA的元转录组分析捕获活体微生物活跃表达的转录,解决DNA分析的缺陷.
研究的目的:
- 为了比较基于DNA (16SrRNA测序) 和基于RNA (转录基因组学) 的分析子宫内膜微生物的技术.
- 评估女性生殖道中的微生物组成和功能潜力.
- 为了更全面地了解子宫内膜微生物群.
主要方法:
- 对49名接受ART的生育年龄妇女进行横截面研究.
- 在分泌期中收集了阴道拭子,子宫内膜刷毛和活检样本.
- 进行了并行的16S rRNA基因测序和元转录组分析.
主要成果:
- 在低生物量子宫内膜样本中观察到16S rRNA基因测序和元转录组学之间的弱相关性.
- 基于RNA的分析在检测某些病原体时提供了更高的分辨率.
- 研究结果表明,潜在的DNA片段或来自下生殖道的乳酸菌细胞,而不是活跃的子宫内膜乳酸菌.
结论:
- 基于DNA和RNA的技术揭示了子宫内膜微生物组成的显著差异.
- 子宫内膜的高宿主RNA和低微生物读数丰度使微生物识别复杂化.
- RNA测序提供了精确的阴道微生物组概况和在失生症中更高的病原体检测,但有限的样本大小限制了概括.
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