用一种新的方法制备的红天然的评估:对结肠癌细胞系的抗炎活性
Xianxiang Xu1, Lin Lin1, Wenjie Ning1
1School of Medicine, Huaqiao University, 269 Chenghua North Road, Quanzhou, 362021, China, hqu.edu.cn.
Mediators of inflammation
|February 5, 2026
概括
新天然 (NIN) 通过激活基碳化合物受体 (AhR) 途径,抑制Wnt/β-catenin信号传递,并减少炎症和癌细胞增殖来缓解性结肠炎 (UC). 这为炎症性肠道疾病提供了新的治疗途径.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病 (IBD),具有与持续性结肠炎症相关的癌症转变的显著风险.
- 天然 (IN),特别是一种新型制剂 (NIN),对炎症和癌症结肠细胞有很大的承诺,但它的机制需要阐明.
研究的目的:
- 用HT-29结肠癌细胞研究NIN对炎症性癌症模型的影响.
- 阐明NIN影响结肠炎症和癌症进展的潜在分子机制.
主要方法:
- 使用HT-29结肠癌细胞系构建了体外炎症模型.
- 将NIN给模型,并评估了促炎性细胞因子 (IL-1β),粘素产生 (MUC2) 和细胞增殖的变化.
- 研究了基受体 (AhR) 和Wnt/β-catenin信号通路的参与,包括关键基因表达 (Rnf43,β-catenin,Axin2,Lgr5).
- 利用一个AhR抗剂 (CH223191) 来确认途径的参与.
主要成果:
- 尼恩降低了IL-1β表达的升高,并减弱了炎症反应.
- 尼恩增加了MUC2的表达,恢复了粘素的分泌,并抑制了HT-29细胞的增殖.
- NIN激活了AhR信号通路,导致Rnf43表达的增加和非酸化β-catenin和Axin2水平的降低.
- 这种激活抑制了Wnt/β-catenin通路,并降低了结直肠癌干细胞标记物Lgr5的表达.
- 尼恩的作用被AhR抗剂CH223191阻断,证实了AhR途径的调解.
结论:
- 在实验室中,NIN有效地减轻性结肠炎相关的炎症和癌症进展.
- 该机制涉及NIN激活AhR信号通路,随后抑制Wnt/β-catenin信号通路.
- 这些发现提供了关于UC炎症癌症转化过程的见解,并突出了NIN作为潜在的治疗剂.
相关概念视频
Cell Lines
10.4K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
10.4K
The Colonization of Land
37.7K
Changes in the environment of the early Earth drove the evolution of organisms. As prokaryotic organisms in the oceans began to photosynthesize, they produced oxygen. Eventually, oxygen saturated the oceans and entered the air, resulting in an increase in atmospheric oxygen concentration, known as the oxygen revolution approximately 2.3 billion years ago. Therefore, organisms that could use oxygen for cellular respiration had an advantage. More than 1.5 years ago, eukaryotic cells and...
37.7K
Inflammatory Response
16.8K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
16.8K
Inflammatory Response II: Inflammatory Exudate and Tissue Repair
7.9K
The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
7.9K
Lossless Lines
576
In electrical engineering, a lossless transmission line is characterized by a purely imaginary propagation constant and a resistive characteristic impedance. The ABCD parameters, which describe the relationship between the input and output voltages and currents, indicate an equivalent π circuit with an imaginary series impedance and a shunt admittance. This results in a transmission line that, when the product of the phase constant (beta) and the length of the line is less than pi, exhibits...
576
Lossy Lines and Overvoltages
361
Transmission-line series resistance and shunt conductance cause three primary effects: attenuation, distortion, and power losses.
Attenuation
When constant series resistance and shunt conductance are present, voltage and current equations are modified. The propagation constant indicates that voltage and current waves consist of both forward and backward traveling components. These waves attenuate as they propagate, with the attenuation factor related to the resistance and conductance. In a...
Attenuation
When constant series resistance and shunt conductance are present, voltage and current equations are modified. The propagation constant indicates that voltage and current waves consist of both forward and backward traveling components. These waves attenuate as they propagate, with the attenuation factor related to the resistance and conductance. In a...
361


