血清β-基酸盐测试的血液溶解,肠炎和脂血症干扰极限
Mark A Cervinski1,2, Macey J Smith2, Gracie Forgues2
1Department of Pathology and Laboratory Medicine, The Geisel School of Medicine at Dartmouth, One Medical Center Dr., Lebanon, NH, United States.
The journal of applied laboratory medicine
|February 5, 2026
概括
这项研究确定了Stanbioβ-Hydroxybutyrate (BOHB) 试验的干扰值,该试验使用的是罗氏Cobas分析仪. 更新的血液溶解,黄和脂血的限值减少了不必要的样本取消,并考虑了分析仪模型的变化.
科学领域:
- 临床化学 临床化学
- 实验室医学 实验室医学
- 分析生物化学 分析生物化学
背景情况:
- 测量β-基酸盐 (BOHB) 对于评估代谢性酸性酶至关重要.
- 斯坦比奥β-基酸液色试剂通常用于BOHB分析.
- 评估血液溶解,黄和脂血的干扰对于测定可靠性至关重要.
研究的目的:
- 为了评估Stanbio BOHB试验的血液溶解,黄和脂血干扰值.
- 为了评估三种不同的罗氏科巴斯分析仪模型的测试性能.
- 为BOHB测试建立最新的,临床相关的干扰极限.
主要方法:
- 创建了患者样本池,使用不同的BOHB度 (0.20,3.00,7.00 mmol/L).
- 在样本池中添加了越来越多的血液溶解物,胆红素或Intralipid®.
- 干扰和BOHB度在罗氏科巴斯c502,c501和c311分析仪上被三次测量.
主要成果:
- 该试验容忍的血液溶解指数高达400 (≤1.00 mmol/L BOHB) 或1010 (>1.00 mmol/L BOHB).
- 在所有测试的BOHB度中,在25 (25 mg/dL bilirubin) 的指数之前,没有观察到菌干扰.
- 根据保守的数据,建立了800的脂血指数极限.
结论:
- 确定了斯坦比奥BOHB试验的最新干扰极限,减少了不必要的样本取消.
- 不同的罗氏科巴斯分析仪模型之间的干扰有所不同,这突显了模型特定验证的重要性.
- 这些发现可以更好地定义临床实验室允许的样品质量限制.
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