一个高分辨率,易于构建的光片显微镜用于亚细胞成像.
John Haug1,2, Seweryn Gałecki1,2,3, Hsin-Yu Lin1,2
1Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, United States.
eLife
|February 5, 2026
概括
我们开发了Altair光片光显微镜 (LSFM),这是一个用于高分辨率亚细胞成像的开源显微镜. 这个系统简化了组装和对齐,使微细的细胞结构可视化.
科学领域:
- 生物物理学的生物物理.
- 显微镜的使用方法
- 细胞生物学 细胞生物学
背景情况:
- 现有的开源光片显微镜 (例如,MesoSPIM,OpenSPIM) 适合大样本,但缺乏对亚细胞细节的分辨率.
- 高分辨率格子光板显微镜 (LLSM) 提供必要的分辨率,但可能是复杂的设置和维护,需要专家知识.
研究的目的:
- 开发一个高分辨率的,开源的,样本扫描光片显微镜,优化用于亚细胞成像.
- 通过简化组装和对齐,同时保持亚细胞分辨率来解决现有平台的局限性.
主要方法:
- 设计了Altair光片光显微镜 (LSFM),具有优化的光路径和定制的底板,以简化对齐和组装.
- 集成精简的光电子和光机械.
- 使用开源软件"导航"系统操作.
主要成果:
- 在266μm视野 (解卷后) 中,分别达到大约235nm和350nm的侧向和轴向分辨率.
- 成功成像了亚衍射光纳米球.
- 哺乳动物细胞中的可视化亚细胞结构,包括微管,活性丝,核和戈尔吉装置.
- 在迁移细胞中展示了动态细胞骨元素的活细胞成像.
结论:
- Altair-LSFM为高分辨率亚细胞成像提供了一个用户友好的开源解决方案.
- 该系统简化了实现纳米分辨率的过程,使先进显微镜更容易获得.
- 验证了细胞组件详细结构和活细胞成像的能力.
关键词:
细胞生物学 细胞生物学人类 人类 人类 人类 人类 人类 人类人类RPE-hTERT细胞光片光显微镜光片光显微镜这里是鼠标鼠标鼠标鼠标鼠标鼠标.在小鼠胚胎纤维细胞中.开源硬件是开源的硬件容量成像成像 容量成像成像更多相关视频
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