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相关概念视频

Subcellular Fractionation01:32

Subcellular Fractionation

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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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Flowsheets are valuable tools in nursing documentation. They enable healthcare professionals to efficiently record and monitor various patient assessments and measurements in a consolidated format.
Here's a closer look at the examples of flowsheets commonly used by nurses:
Graphic Sheet Documentation:
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Movement Joints in Buildings01:27

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Movement joints in buildings are essential design elements that accommodate inevitable motions caused by various factors such as temperature changes, moisture content variations, and structural deflections. These motions, if not considered in design and construction, can lead to unsightly or dangerous damage. Movement joints are incorporated in different forms to manage these stresses and allow materials to move without causing distress.
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Building stones, essential materials for construction, are extracted from natural rock deposits and processed into specific forms and dimensions suitable for various building applications. These stones are broadly classified into three types based on their geological formation: igneous, sedimentary, and metamorphic.
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Building separation joints divide large or complex building structures into smaller, discrete units that can move independently. These joints are categorized into three types: volume-change joints, settlement joints, and seismic separation joints.
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Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development
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一个高分辨率,易于构建的光片显微镜用于亚细胞成像.

John Haug1,2, Seweryn Gałecki1,2,3, Hsin-Yu Lin1,2

  • 1Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, United States.

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概括

我们开发了Altair光片光显微镜 (LSFM),这是一个用于高分辨率亚细胞成像的开源显微镜. 这个系统简化了组装和对齐,使微细的细胞结构可视化.

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细胞生物学 细胞生物学人类 人类 人类 人类 人类 人类 人类人类RPE-hTERT细胞光片光显微镜光片光显微镜这里是鼠标鼠标鼠标鼠标鼠标鼠标.在小鼠胚胎纤维细胞中.开源硬件是开源的硬件容量成像成像 容量成像成像

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科学领域:

  • 生物物理学的生物物理.
  • 显微镜的使用方法
  • 细胞生物学 细胞生物学

背景情况:

  • 现有的开源光片显微镜 (例如,MesoSPIM,OpenSPIM) 适合大样本,但缺乏对亚细胞细节的分辨率.
  • 高分辨率格子光板显微镜 (LLSM) 提供必要的分辨率,但可能是复杂的设置和维护,需要专家知识.

研究的目的:

  • 开发一个高分辨率的,开源的,样本扫描光片显微镜,优化用于亚细胞成像.
  • 通过简化组装和对齐,同时保持亚细胞分辨率来解决现有平台的局限性.

主要方法:

  • 设计了Altair光片光显微镜 (LSFM),具有优化的光路径和定制的底板,以简化对齐和组装.
  • 集成精简的光电子和光机械.
  • 使用开源软件"导航"系统操作.

主要成果:

  • 在266μm视野 (解卷后) 中,分别达到大约235nm和350nm的侧向和轴向分辨率.
  • 成功成像了亚衍射光纳米球.
  • 哺乳动物细胞中的可视化亚细胞结构,包括微管,活性丝,核和戈尔吉装置.
  • 在迁移细胞中展示了动态细胞骨元素的活细胞成像.

结论:

  • Altair-LSFM为高分辨率亚细胞成像提供了一个用户友好的开源解决方案.
  • 该系统简化了实现纳米分辨率的过程,使先进显微镜更容易获得.
  • 验证了细胞组件详细结构和活细胞成像的能力.