相关实验视频

Updated: Feb 7, 2026

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Published on: October 22, 2012

通过植物免疫NADPH氧化酶利用化学发光来可视化和量化可塑性ROS

Miki Yoshioka¹, Hirofumi Yoshioka²

¹Laboratory of Plant Immunology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Aichi, Japan.

Methods in molecular biology (Clifton, N.J.)

|February 5, 2026

概括

活性氧物种 (ROS) 对于植物免疫力至关重要. 这项研究引入了一种检测植物ROS爆发的新方法,有助于研究植物对病原体的防御机制.

科学领域:

植物生物学植物生物学
分子植物病理学分子植物病理学
生物化学生物化学

背景情况:

活性氧物种 (ROS) 在植物天生的免疫力中起着关键作用.
NADPH氧化酶 (RBOH) 产生ROS,如超氧化物 (O₂^-) 和过氧化 (H₂O₂),作为对病原体识别的反应.
在 *Nicotiana benthamiana* 和 *Solanum tuberosum* 中,特定的 RBOH 蛋白与在模式触发免疫 (PTI) 和效应因子触发免疫 (ETI) 期间的 ROS 生产有关.

研究的目的:

开发和介绍一种用于检测无形成性ROS的新方案.
调查土豆STRBOHC和StCDPK5在*N. benthamiana*中的共同表达,以检测ROS.

主要方法:

使用L-012衍生化学发光来检测ROS.
在 *Nicotiana benthamiana* 叶子中*Solanum tuberosum* StRBOHC 和 StCDPK5 的经过农细菌介导的共同表达.

主要成果:

成功建立了一个基于化学发光的协议,用于检测可塑性ROS.
证明了使用*N. benthamiana*作为研究土豆RBOH和CDPK相互作用的模型系统的可行性.

结论:

开发的协议为研究植物免疫力中的ROS动态提供了一种敏感的方法.
这项研究有助于进一步研究RBOH和依赖的蛋白激酶在植物防御反应中的作用.

关键词:

形ROS是指发生形的ROS.这是一种依赖的蛋白质激酶.化学发光的光泽 L-01212 这是什么意思?在NADPH氧化酶.在RBOH中,RBOH就是RBOH.

更多相关视频

Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells

Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells

Published on: July 5, 2024

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Published on: November 25, 2022

相关概念视频

The Apoplast and Symplast

The Apoplast and Symplast

Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

What is the Immune System?

What is the Immune System?

Plant Hormones

Plant Hormones

Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.

Tonicity in Plants

Tonicity in Plants

Tonicity describes the capacity of a cell to lose or gain water. It depends on the quantity of solute that does not penetrate the membrane. Tonicity delimits the magnitude and direction of osmosis and results in three possible scenarios that alter the volume of a cell: hypertonicity, hypotonicity, and isotonicity. Due to differences in structure and physiology, tonicity of plant cells is different from that of animal cells in some scenarios.

Plant Cell Wall

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.