基于生物发光的生长量化对植物病原菌的Pseudomonas syringae pv. 番茄 DC3000 DC3000 的时间
Yuga Fujinawa1, Yijia Yan2, Hirofumi Nakagami2
1Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Methods in molecular biology (Clifton, N.J.)
|February 5, 2026
概括
使用pBJ矢量系统进行生物发光标记,创建一个稳定,明亮的Pseudomonas syringae pv. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 这种方法准确地量化了植物中的细菌生长,而不会影响健康.
科学领域:
- 微生物学 微生物学
- 植物病理学 植物病理学
- 分子生物学分子生物学
背景情况:
- 生物发光标签对于研究植物病原菌感染至关重要.
- 尽量减少基因修饰对细菌健康的影响是生物发光研究的关键.
- pBJ 矢量系列为 Pseudomonadota.的生物发光标记提供了一个全集系统.
研究的目的:
- 提出基于生物发光的细菌生长分析的协议.
- 详细说明使用一种新型生物发光的Pseudomonas syringae pv. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株. 番茄DC3000 (Pto) 菌株.
- 为了能够准确地量化植物中的细菌.
主要方法:
- 开发用于可诱导的Tn7介导转换的pBJ向量序列.
- 在Pseudomonas syringae pv.中插入luxCDABE luciferase操作子 番茄DC3000 (Pto) 番茄DC3000 (Pto) 番茄DC3000 (Pto) 番茄DC3000 (Pto) 番茄DC3000 (Pto) 番茄DC3000 (Pto) 番茄DC3000 (Pto) 番茄DC3000 (Pto) 番茄DC3000 (Pto) 番茄DC3000 (Pto) 番茄
- 在Arabidopsis thaliana和Marchantia polymorpha.中建立基于生物发光的生长分析.
主要成果:
- 由此产生的Pto-lux菌株表现出稳定,强烈的生物发光.
- 在植物感染期间保持细菌健康.
- 生物发光测定准确量化植物细菌标位在四个log动态范围.
结论:
- 该pBJ向量系统提供了一个有效的工具,用于生物发光标记的植物病原菌.
- 普托卢克斯能够对植物中的细菌感染进行强大而准确的监测.
- 对Arabidopsis thaliana和Marchantia polymorpha感染模型建立了协议.
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