斯洛波克:一个自动化的金门克隆工作流程,用于Opentron OT-2和Flex
Koray Malcı1,2, Fankang Meng1,2, Henri Galez3
1Department of Bioengineering, Imperial College London, London SW7 2AZ, U.K.
ACS synthetic biology
|February 5, 2026
概括
Slowpoke使用开源液体处理器自动化了金门克隆,简化了合成生物学的DNA组装. 这种用户友好的工作流程提高了研究实验室的标准化和高通量能力.
科学领域:
- 合成生物学 合成生物学
- 分子生物学分子生物学
- 生物工程是生物工程.
背景情况:
- 在合成生物学中,DNA组装至关重要,越来越需要自动化,标准化和高吞吐量方法.
- 现有的解决方案往往缺乏适用于不同实验室环境的可访问性或灵活性.
- 对无错误和高效的DNA组装的需求推动了自动化工作流程的创新.
研究的目的:
- 为了介绍Slowpoke,一个用户友好和灵活的自动化工作流程,用于金门DNA组装.
- 为了适应这个工作流程的流行的,低成本的,开源的液体处理平台 (Opentrons OT-2和Flex).
- 开发一个图形用户界面 (GUI),用于简化协议生成.
主要方法:
- 使用Opentrons OT-2和Flex进行DNA克隆,大肠杆菌转化,涂层和殖民地PCR的自动执行.
- 开发一个免费的,基于Web的GUI (https://slowpoke.streamlit.app/),通过文件上传创建协议.
- 使用MoClo酵母工具包 (YTK) 和SubtiToolKit (STK) 验证基本和高通量组件.
主要成果:
- 实现了高组装效率:基本结构的17/17 (YTK/OT-2),11/12 (YTK/Flex) 和8/13 (STK/OT-2).
- 在Flex (55/57正确) 上使用YTK兼容部件成功组装了六部分结构的高通量组装.
- 在不同的工具包和自动化平台中证明了强度和适应性.
结论:
- 斯洛普克为金门克隆提供了一个可访问,模块化和自动化的解决方案.
- 该工作流显著提高了合成生物学研究中的标准化和高吞吐量能力.
- 这个免费可用的套件 (https://github.com/Tom-Ellis-Lab/Slowpoke) 支持在研究实验室中更广泛地采用.
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