DISCERN:通过双Aptamer启动的传感电路工程纳米酶用于白血病干细胞表型化
1Department of Biology, School of Basic Medical Sciences, Guizhou Medical University, Guian New District, Guiyang 561113, P.R. China.
ACS sensors
|February 5, 2026
概括
我们开发了DISCERN,这是一种敏感的平台,用于瘤干细胞 (LSC) 现型化,用于急性髓性白血病 (AML). 这项技术改善了最小残留疾病监测和复发预测.
科学领域:
- 生物技术是生物技术.
- 纳米技术纳米技术
- 血液学 血液学 血液学
背景情况:
- 白血病干细胞 (LSC) 现型化对于在急性髓性白血病 (AML) 中进行最小残留疾病 (MRD) 监测至关重要.
- 目前的方法缺乏敏感性和特异性来检测罕见的抗化疗LCS (crLSCs) 或识别干状状态.
- 这限制了AML患者有效的治疗评估和复发预测.
研究的目的:
- 开发一个高度敏感和特定的平台,用于LSC表型.
- 为了能够可靠地检测罕见的crLSCs和识别干状表型.
- 推进MRD监测,风险分层和AML的精密治疗.
主要方法:
- 开发DISCERN (通过工程纳米酶启动的双Aptamer传感电路),一个测色平台.
- 采用双亚普坦体系统,针对同定位的表面标记物 (CD33和CD123) 进行特定的识别.
- 采用局部催化级联,包括滚动圆放大 (RCA) 和PCN-222 (Fe) 纳米酶用于信号放大.
主要成果:
- 获得了异常灵敏度,检测极限低于10细胞/毫升.
- 证明了DISCERN在白血病细胞中追踪表型可塑性的能力.
- 在白血病异种移植模型中成功识别了类似茎的子集.
结论:
- DISCERN提供超敏感的高特异性的LSC表型.
- 该平台具有成本效益,强大,并适合临床翻译.
- 在AML风险分层,复发预测和指导精确治疗方面,DISCERN显示出前景.
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