通过双提取驱动的柔性微针传感器来积极检测黑色素瘤
Min Wu1, Songlong Jiao2, Lei Liu1
1School of Mechanical Engineering, Southeast University, Nanjing, 211189, People's Republic of China.
Biosensors & bioelectronics
|February 5, 2026
概括
这项研究引入了一种双提取灵活的微针生物传感器,用于早期发现黑色素瘤. 可穿戴式传感器增强了间歇性液体的收集,用于高度敏感的实时miRNA检测.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 可穿戴式生物传感器
背景情况:
- 可穿戴的微针 (MN) 生物传感器对间歇性液体 (ISF) 中的微侵入性生物标志物检测充满希望.
- 临床翻译受到低流体收集效率,皮肤刺激和长期稳定性差的阻碍.
- 早期疾病诊断需要敏感和可靠的检测方法.
研究的目的:
- 开发一个完全集成的双提取驱动的柔性微针 (DFMN) 传感器,用于积极检测黑色素瘤相关的miRNA.
- 克服现有的可穿戴生物传感器的局限性,以提高临床适用性.
- 为了实现高灵敏度的生物标记物的实时无线检测.
主要方法:
- 制造一个DFMN传感器,集成水凝MN,空心Au-AgNP功能化的柔性电极和一个无线模块.
- 通过被动扩散 (水凝膨胀) 和主动输送 (反向离子泳) 的协同应用,提高ISF提取率.
- 实现人工神经网络算法用于信号处理和可靠性增强.
主要成果:
- 在5分钟内,DFMN传感器实现了ISF提取体积的1.6倍增加.
- 实时无线检测miRNA-221的超低检测极限 (LOD) 为43 aM.
- 人工神经网络算法导致高相关系数 (R^2) 0.985可靠的检测.
结论:
- 开发的DFMN传感器在可穿戴生物传感技术方面取得了重大进展.
- 双提取机制和人工智能集成提高了生物标志物检测的灵敏度,效率和可靠性.
- 这项技术在智能医疗保健,人机交互和人工智能驱动的诊断方面具有广泛的应用.
相关概念视频
ATP Driven Pumps I: An Overview
9.9K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
9.9K
Xylem and Transpiration-driven Transport of Resources
26.8K
The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.
26.8K
ATP Driven Pumps II: P-type Pumps
6.4K
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
6.4K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
1.3K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.3K
ATP Driven Pumps III: V-type Pumps
4.9K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
4.9K
Extraction: Effects of pH
1.4K
Consider a neutral form of an amine, B, with a partition coefficient, K, in a liquid mixture containing organic and aqueous phases. The pH of the aqueous phase affects the charge on acidic and basic solutes, and the charged form is usually more soluble in the aqueous phase. Suppose the conjugate acid form of the amine is soluble only in the aqueous phase while the base form is soluble in both phases. Then the distribution coefficient, D, can be given as the ratio of amine concentration in the...
1.4K


