在体内通过智能手机进行细胞内氧气测量,读取内源性Protoporphyrin IX延迟光的读数
Protik Chandra Biswas1, Jason R Gunn1, Tayyaba Hasan2
1Thayer School of Engineering, Dartmouth College, Hanover, NH, United States.
Biosensors & bioelectronics
|February 5, 2026
概括
这项研究引入了一种基于智能手机的新型系统,用于实时,体内测量线粒体氧张力. 该技术提供了一种灵敏,快速的方法来评估组织代谢和疾病状态.
科学领域:
- 生物医学工程 生物医学工程
- 细胞的新陈代谢
- 光学传感传感器是什么?
背景情况:
- 与血液和相比,线粒体氧气张力 (pO2) 的直接测量可以更好地了解组织代谢.
- 现有的测量pO2的方法往往很慢,或者需要复杂的设备.
- 线粒体利用Protoporphyrin IX (PpIX),它表现出对氧气敏感的光.
研究的目的:
- 开发一种新,快速和灵敏的平台,用于体内测量组织氧气部分压力.
- 利用智能手机CMOS相机技术来量化依赖氧气的光信号.
- 通过细胞水平的代谢数据,实现疾病检测和监测的新方法.
主要方法:
- 设计了一个平台系统,利用智能手机CMOS摄像头的滚动快门读数.
- 从线粒体内内源性Protoporphyrin IX (PpIX) 来测量时间解析的延迟光 (DF).
- 利用滚动快门作为一个时间隔离的快门来采样DF强度,消除了对专门的高速摄像机的需求.
主要成果:
- 在广泛的pO2范围 (0-160 mmHg) 和PpIX度 (0.5-10 μM) 的组织幻象中验证了仪器性能.
- 实现了可调节,快速响应时间 (1-3.5μs),显著优于基于电极的系统.
- 在体内成功测试,以在不同吸入氧气条件下评估皮肤组织的pO2 (正常氧,高氧,低氧).
结论:
- 开发的基于智能手机的系统提供了一种新,快速和灵敏的方法,用于直接在体内测量组织氧气局部压力.
- 这种技术通过利用现有的智能手机硬件简化了氧气传感.
- 该平台具有促进细胞代谢研究,疾病诊断和治疗监测的巨大潜力.
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