一个响应pH的探针用于硫化物离子检测和细胞内pH/硫化物传感
Xiao-Hui Wang1, Jun-Yu Luan1, Zhi-Shi Feng1
1State Key Laboratory of Advanced Optical Polymer and Manufacturing Technology, Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|February 5, 2026
概括
硫-pH是一种用于检测性条件和硫化物离子 (S2-) 的新型光探针. 它在真实水样和活细胞中提供快速,稳定和特定的检测.
科学领域:
- 分析化学 分析化学
- 化学传感器 化学传感器
- 生物医学工程 生物医学工程
背景情况:
- 轻微性环境需要敏感的检测方法.
- 硫化物离子 (S2-) 在各种化学和生物过程中起着至关重要的作用.
- 现有的S2检测方法可能会受到干扰,缺乏特异性.
研究的目的:
- 开发一种pH敏感的光探针,Sul-pH,用于检测轻微性环境.
- 通过将探测器响应与S2-水解相关联,使硫化物离子 (S2-) 的定量检测成为可能.
- 评估探测器在现实世界样本和生物系统中的性能.
主要方法:
- 基于基于ICT的分子设计的硫-pH的设计和合成,采用循环合金光体.
- 探测器pH响应性行为的表征,包括光强度变化和视觉颜色变化.
- 探针反应与硫化物离子 (S2-) 水解的相关性,用于定量分析.
- 在水样和活细胞中评估探针的特异性,响应时间,稳定性和性能.
主要成果:
- 硫-pH在7-10的pH范围内表现出显著的光增强 (近250倍).
- 探头显示出快速响应 (<6秒) 和出色的pH循环稳定性和光稳定性.
- 硫-pH通过检测来自S2-水解的性条件来准确量化硫化 (S2-) 离子,而不会受到HS-的干扰.
- 探测器在真实水样和活细胞中成功检测了S2和pH.
结论:
- 硫-pH是一种高效的光探测器,用于轻微性环境和硫化物离子 (S2-) 的敏感和特定检测.
- 它的快速反应,稳定性和在复杂样品和生物系统中的适用性使其成为化学传感和生物监测的宝贵工具.
- 硫酸为监测细胞内pH动态和硫化物离子波动提供了一种可靠的方法.
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