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1,2-二醇的代谢是通过酸面粉中的异种发酵乳酸菌发生的

Qin Li¹, Vi D Pham², Michael Gänzle³

¹University of Alberta, Dept. of Agricultural, Food and Nutritional Science, Edmonton, Canada; College of Biological and Food Engineering, Hubei Minzu University, Enshi, Hubei, PR China.

International journal of food microbiology

|February 5, 2026

概括

乳杆菌将乳酸转化为1,2-二醇,维持细胞活力. 这项研究探讨了各种乳酸菌中的这种途径,揭示了菌株特定的生产和基因表达模式,特别是在静止阶段.

科学领域:

微生物学微生物学
代谢工程是代谢工程.
食品生物技术食品生物技术

背景情况:

乳杆菌利用乳酸到1,2-propanediol通路进行长期生存.
乳酸菌中这种代谢途径的调节尚未完全理解.
这一途径对于长时间的静止阶段细胞存活至关重要.

研究的目的:

在异质发酵乳酸菌中研究1,2-二醇代谢途径.
专注于lentilactobacilli,levilactobacilli和furfurilactobacilli. 这三种类型的细菌是最常见的.
分析影响途径表达和产品形成的因素.

主要方法:

培养的特定乳杆菌菌株 (Lentilactobacillus buchneri,Furfurilactobacillus spp.) 进行培养. 在修改后的MRS (mMRS) 和酸面粉介质中.
测量了1,2-二醇和曼尼醇的生产.
在不同的pH,乳酸和生长阶段分析了aldA (乳甲基脱酶) 和pduC (二醇利用酶) 的基因表达.

主要成果:

黄菌种 (Lentilactobacillus buchneri) 产生的高含量1,2-二醇;黄菌种 (Furfurilactobacillus spp.) 产生的高含量二醇;黄菌种 (Furfurilactobacillus spp.) 产生的高含量二醇. 产生了曼尼托尔.
Sorghum 酵母面的发酵导致了 Ff. 中 1,2-propanediol 的增加. 在谷物C5和Ff. 罗西亚. 罗西亚. 罗西亚. 罗西亚.
静止阶段aldA表达增加,与持续的1,2-propanediol转化相关;pduC表达在各种条件中是一致的.

结论:

乳酸菌在1,2-propanediol代谢中表现出代谢的多功能性.
在静止阶段,ALDA基因上调,支持长时间的乳酸转化.
研究结果为食品生物技术的潜在应用提供了洞察力.

关键词:

不同发酵的乳酸菌.乳甲酸脱基酶的使用方法牛乳杆菌 (Lactobacillus) 是一种细菌.Pickles Pickles Pickles 是一种葡萄类的蔬菜.化物化剂是一种化物化剂.酸面粉是一种酸面粉.

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