暴露于flocoumafen会诱导斑马鱼 (Danio rerio) 的骨发育毒性和神经毒性
Tianyi Chen1, Fengjun Fan2, An Cheng2
1Zhejiang Key Laboratory of Drug Prevention and Control Technology, The Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou, Zhejiang Province, People's Republic China; Hangzhou Public Security Bureau West Lake Scenic area Branch, Hangzhou, Zhejiang Province, People's Republic China.
Toxicology letters
|February 5, 2026
概括
弗洛库马芬 (Flocoumafen,FCF) 是一种抗凝固剂杀虫剂,在斑马鱼胚胎中引起骨和神经损伤,影响生存和行为. 阿斯塔丁 (ASTA) 提供了对FCF毒性的部分保护.
科学领域:
- 环境毒理学环境毒理学
- 水生毒理学 水生毒理学
- 杀虫剂生态毒理学 杀虫剂生态毒理学
背景情况:
- 像Flocoumafen (FCF) 这样的第二代抗凝固剂杀剂 (SGAR) 构成环境风险.
- 对于FCF对水生生物的影响,有有限的毒理学数据.
- 斑马鱼胚胎是评估发育毒性的敏感模型.
研究的目的:
- 调查FCF对斑马鱼胚胎的毒理影响.
- 阐明FCF引起的发育异常背后的机制.
- 评估阿斯塔克桑丁 (ASTA) 对FCF毒性的潜在保护作用.
主要方法:
- 斑马鱼胚胎暴露在不同度 (0.2,0.4,0.8 mg/L) 的FCF中.
- 包括生存,形态和行为在内的发育终点被评估到受精后120小时 (hpf).
- 分析了基因表达 (Notch信号通路),氧化应激标记 (ROS,SOD,CAT,MDA) 和神经元完整性.
主要成果:
- 暴露于FCF显著降低了生存率,并诱导了面和骨异常 (身体长度缩短,脊椎曲,骨矿化不完全).
- 观察到神经元损失和显著的行为缺陷 (减少运动能力,活动,转动能力).
- FCF破坏了Notch信号通路的基因表达,增加了氧化应激 (ROS,SOD,CAT,MDA) 和阿斯丁 (ASTA) 提供了部分缓解.
结论:
- 在斑马鱼胚胎中,FCF诱导了显著的骨和神经毒性,与氧化应激和Notch通路中断有关.
- 观察到的发育和行为异常凸显了FCF对水生生物的生态毒理风险.
- 这些发现支持对FCF的监管评估,并建议阿斯塔丁作为潜在的对策.
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