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Updated: Feb 7, 2026

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通过无氧/有氧/无毒策略将外源转换为内源电子捐赠驱动的脱化:关键驱动因素,性能增强和微生物社区动态

Shao Zhu¹, Shenbin Cao², Yongzhen Peng¹

¹National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, PR China.

Environmental research

|February 5, 2026

概括

这项研究通过内源性脱增强了废水中的去除. 它成功地丰富了Candidatus Competibacter,通过利用储存的聚合物,如糖原,作为没有外部碳的电子捐赠者来提高效率.

科学领域:

环境微生物学环境微生物学
废水处理工程废水处理工程
生物技术是生物技术.

背景情况:

从低碳化 (C/N) 的城市废水中有效地去除是一种重大挑战.
无氧/有氧/无氧 (A/O/A) 过程中的内源性脱 (ED) 提供了碳和能源节约的潜力,但面临着启动和微生物丰富的困难.
现有的方法往往需要外部碳来源,增加运营成本和复杂性.

研究的目的:

证明在低C/N废水的A/O/A测序批量反应堆 (SBR) 中成功过渡到内源电子捐赠驱动的脱化.
优化操作条件,包括低溶解氧 (DO) 和延长无氧持续时间,以丰富关键功能微生物.
研究不同内部碳来源和微生物群落在实现高效的去除中的作用.

主要方法:

在优化低DO (0.5-1.0 mg/L) 和延长无氧 (4小时) 条件下运行A/O/A SBR.
在无氧阶段 (最佳时间为90分钟) 监测聚酸酸盐 (PHA) 积累的情况.
微生物社区分析使用16SrRNA测序来识别主导的功能性微生物.
在脱过程中使用的糖原和其他内部碳来源 (EPS,代谢物) 的量化.

主要成果:

实现了Candidatus Competibacter的显著丰富 (从0.96%到35.42%的相对丰度),被认为对ED至关重要.
在没有外部碳添加的情况下,提升了从60.2%到83.2%的除效率.
在延长的无氧阶段中,鉴定出糖原是主要的电子捐赠者,补充了PHA,EPS和细胞代谢物.
证明低DO条件将有氧PHA消耗量降至最低,并将其保存为脱化.

结论:

低DO和延长无氧条件的结合策略成功丰富了脱糖体积聚生物 (DGAO),特别是Candidatus Competibacter.
这种方法可以通过促进内部碳来源的利用来有效地去除,从而消除了对外部碳添加的需求.
该方法减少了污泥的产生,并为处理低C/N的城市废水提供了可持续的解决方案.

关键词:

一个A/O/A的过程过程.脱化糖原积聚生物 (DGAO) 是一种内源性脱化是一种内源性脱化.细胞内部的碳储存.低C/N废水的废水

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