相关实验视频

Updated: Feb 7, 2026

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Published on: September 23, 2021

独特的蛋白质和糖化特征区分A549瘤和BEAS-2B非瘤细胞系衍生的小细胞外囊泡

Mirjam Balbisi¹, Tamás Langó², Virág Nikolett Horváth³

¹MTA-HUN-REN TTK Lendület (Momentum) Glycan Biomarker Research Group, HUN-REN Research Centre for Natural Sciences, Magyar tudósok körútja 2, H-1117, Budapest, Hungary; Semmelweis University Doctoral School, Üllői út 26, H-1085, Budapest, Hungary.

Molecular & cellular proteomics : MCP

|February 5, 2026

概括

使用N-糖蛋白组学和糖氨基甘油 (GAG) 分析对细胞外囊泡 (EVs) 的表征揭示了不同的分子特征. 这种方法增强了对EV起源和作为癌症生物标志物的潜力的理解.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

细胞外囊泡 (EVs) 对于细胞间通信至关重要,并具有作为癌症生物标志物的潜力.
目前的EV研究往往忽略了详细的糖化分析,限制了全面的分子表征.
蛋白质糖化,一个关键的翻译后修饰,在EV研究中表现不足.

研究的目的:

调查和比较小EVs (sEVs) 的蛋白质组,N-糖蛋白组和氏素/皮质硫酸盐 (CS/DS) 糖氨酸甘油 (GAG) 档案.
为了分析从A549肺腺癌和BEAS-2B非瘤源的上皮细胞中衍生出的sEVs.
确定补充糖分和GAG分析对表征sEV异质性的有用性.

主要方法:

对SEVs的蛋白质组分析.
对sEVs的N-glycoproteomic分析.
孔德罗/皮质硫酸盐 (CS/DS) 糖氨基甘氨酸 (GAG) 对SEVs进行分析.
使用主要组件分析和等级聚类进行比较分析.

主要成果:

蛋白质组,N-糖蛋白组和GAG配置文件有效地区分了sEV来源.
A549 sEVs在细胞周期,DNA修复和新陈代谢蛋白中表现出丰富,而BEAS-2B sEVs在与免疫相关的过程中表现出丰富.
在A549 sEV中,N-糖蛋白组学揭示了转向复杂N-糖的转变.
确定了五种差异表达的CS蛋白质甘氨酸,A549 sEVs中的CS/DS含量显著更高.

结论:

N-糖蛋白组学和GAG分析对于对sEVs的敏感分子特征有价值.
这些互补分析揭示了不同细胞类型的sEV之间明显的分子差异.
这些发现为未来癌症研究中EV生物标志物研究提供了基础.

关键词:

在N-glycoproteomics中使用.康德罗素/皮肤硫酸盐细胞外囊泡细胞外囊泡质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量蛋白质组学蛋白质组学

更多相关视频

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

相关概念视频

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Cancer Stem Cells and Tumor Maintenance

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...